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Merck

L2380

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

lyophilized powder

Szinonimák:

Bandeirea simplicifolia agglutinin, BS-I

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352202
NACRES:
NA.32

Forma

lyophilized powder

Minőségi szint

hatékonyság

<20 μg per mL agglutination activity (using human blood group B erythrocytes)

összetétel

Protein, ≥90% biuret

szennyeződések

salt, free

tárolási hőmérséklet

2-8°C

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Biokémiai/fiziológiai hatások

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Egyéb megjegyzések

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Elkészítési megjegyzés

Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).

Analízis megjegyzés

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


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