H3159

Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Szinonimák: Anti-HD2, Anti-KDAC2, Anti-RPD3, Anti-YAF1
• MDL-szám: MFCD02096544
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: ARR; IHC (p); IP; WB
• klón: polyclonal
• faj reaktivitás: mouse, human
• citations: 24
• technika/technikák: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections; immunoprecipitation (IP): 50 μg using whole lysate of NIH3T3 cells; microarray: suitable; western blot: 1:20,000 using nuclear extract from human HeLa cells

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: IgG fraction of antiserum
• antitest terméktípus: primary antibodies
• klón: polyclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen 55 kDa
• faj reaktivitás: mouse, human
• technika/technikák: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections; immunoprecipitation (IP): 50 μg using whole lysate of NIH3T3 cells; microarray: suitable; western blot: 1:20,000 using nuclear extract from human HeLa cells
• UniProt elérési szám: Q92769
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... HDAC2(3066) ; mouse ... Hdac2(15182)

Leírás

Általános leírás

Histone Deacetylase (HDACs) have been described as six or seven different groups in mammals. HDAC1, HDAC2 and HDAC3 are like yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are like yeast Hda1 (Histone Deacetylase 1) protein.

Egyediség

Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin.

Immunogén

synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively).

Alkalmazás

Anti-Histone Deacetylase 2 (HDAC2) antibody has been used:

• in immunoblotting and dot blot
• in immunoprecipitation
• in immunohistochemistry
• in enzyme linked immunosorbent assay (ELISA)
• in western blotting
• in chromatin immunoprecipitation(ChiP)

Biokémiai/fiziológiai hatások

Histone acetylation is a dynamic process whose levels are determined by the net activities of Histone acetyl transferase (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosome condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

Fizikai forma

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable