G5507

Galactosyltransferase from bovine milk

lyophilized powder

• Szinonimák: Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase
• CAS-szám: 9031-68-9
• Enzyme Commission szám: 2.4.1.22 (BRENDA, IUBMB)
• MDL-szám: MFCD00131122
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• Forma: lyophilized powder
• Minőségi szint: 200
• specifikus aktivitás: 3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)
• összetétel: Protein, 35-65% modified Warburg-Christian
• szennyeződések: ≤10 % protein α-lactalbumin
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Research area: Cell signalling. Galactosyltransferase(GalT) is found in both prokaryotes and eukaryotes.Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.

Alkalmazás

Galactosyltransferase from bovine milk has been used:

• in the generation of hyper-galactosylated variant of IgG1
• in β -1,4-galactosyltransferase assay rice root cell extracts
• in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Biokémiai/fiziológiai hatások

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.

Galactosyltransferase from bovine milk requires Mn²⁺ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various disease states.Galactosyltransferase(GalT) functions either as a soluble enzyme or as membrane-bound moieties.

Kapcsolódás

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Egység definíció

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Fizikai forma

Lyophilized powder containing Tris, EDTA, and (NH₄)₂SO₄

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)