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Merck

EDEGLY

Sigma-Aldrich

Enzymatic Protein Deglycosylation Kit

Szinonimák:

Glycosylation Release Kit

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352202
NACRES:
NA.32

konjugátum

(N-linked)
(O-linked)

Minőségi szint

kiszállítva

wet ice

tárolási hőmérséklet

2-8°C

Általános leírás

Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.

Alkalmazás

Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.
Enzymatic protein deglycosylation kit has been used in:
  • protein deglycosylation
  • deglycosylation of tissue factor pathway inhibitor (TFPI) α and β
  • deglycosylation of native exo-β-(1,3)-glucanase EXG1

Tulajdonságok és előnyök

Enzymatic protein deglycosylation kit has the following features:
  • two or more mg of glycoprotein is deglycosylated
  • single reaction at neutral pH
  • native & denaturing procedures
  • no degradation of protein
  • O-linked sugars containing polysialic acid are eliminated
  • control glycoprotein provided

Kit Components Only

Product No.
Leírás

  • PNGase F 1 mL/vial

  • O-Glycosidase 20 μL

  • α-2(3,6,8,9)-Neuraminidase 20 μL

  • Fetuin Control .5 mg

  • 5× Reaction Buffer .2 mL

  • Denaturation Solution .1 mL

  • Triton X-100 .1 mL

  • β-1→4-Galactosidase, positionally specific 20 μL

  • β-N-Acetylglucosaminidase 20 μL

Összes mutatása (9)

kapcsolódó termék

Product No.
Leírás
Árazás

Figyelmeztetés

Danger

Veszélyességi osztályok

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Tárolási osztály kódja

10 - Combustible liquids

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number

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Dokumentumtár megtekintése

Jonathan Trapala et al.
Protein expression and purification, 176, 105718-105718 (2020-08-11)
Exo-inulinases are versatile enzymes that have gained attention in recent years due to their ability to hydrolyze linear and branched polyfructose chains found in inulines. Agavin, a branched inulin, is found in Agave plant, the raw matter to produce tequila.
Roman Szabo et al.
PloS one, 13(4), e0194660-e0194660 (2018-04-05)
Prostasin (CAP1/PRSS8) is a glycosylphosphatidylinositol (GPI)-anchored serine protease that is essential for epithelial development and overall survival in mice. Prostasin is regulated primarily by the transmembrane serine protease inhibitor, hepatocyte growth factor activator inhibitor (HAI)-2, and loss of HAI-2 function
Noémie Caillot et al.
The Journal of allergy and clinical immunology, 140(3), 759-770 (2016-12-15)
Eligibility to immunotherapy is based on the determination of IgE reactivity to a specific allergen by means of skin prick or in vitro testing. Biomarkers predicting the likelihood of clinical improvement during immunotherapy would significantly improve patient selection. Proteins were differentially
Angela Mika et al.
PLoS neglected tropical diseases, 5(9), e1331-e1331 (2011-10-08)
Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families
Guillain-Barre syndrome: First description of a snake envenomation aetiology
Neil J, et al.
Journal of Neuroimmunology, 242(1-2), 72-77 (2012)

Cikkek

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.

Protein modifications are crucial for disease study. Analysis methods are key.

Összes mutatása

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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