J774A.1 Cell Line from mouse

91051511, mouse blood, Not specified

• Szinonimák: J774 A1 Cells, J774.A1 Cells, J774A1 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• product name: J774A.1 Cell Line from mouse, 91051511
• biológiai forrás: mouse blood
• leírás: Mouse BALB/c monocyte macrophage
• növekedési mód: Semi-adherent
• kariotípus: Not specified
• morfológia: Not specified
• termékek: Interleukin 1 (IL-1), lysozyme
• receptorok: Immunoglobulin, complement
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Mouse BALB/c monocyte macrophage

Sejtvonal leírása

Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.

Alkalmazás

J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.

Metabolic studies

Táptalaj

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm² Incubate at 37°C; 5% CO₂. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.

Egyéb megjegyzések

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