POLYDIC-RO

Poly[d(I-C)]

lyophilized, pkg of 10 U (10108812001 [A₂₆₀ units]), pkg of 50 U (11219847001 [A₂₆₀ units])

• Szinonimák: Poly-deoxy-inosinic-deoxy-cytidylic acid
• UNSPSC kód: 12352200

Tulajdonságok

• leírás: Poly-deoxy-inosinic-deoxy-cytidylic acid
• Minőségi szint: 100
• form: lyophilized
• molekulatömeg: 28814 bp
• kiszerelés: pkg of 10 U (10108812001 [A₂₆₀ units]); pkg of 50 U (11219847001 [A₂₆₀ units])
• gyártó/kereskedő neve: Roche
• szín: white
• mp: 54 °C (NaCl, 0.1 M, pH 7.0)
• oldhatóság: water: soluble
• abszorbancia arány: A_{290/260 nm} 0.24-0.32; A_{280/260 nm} 0.61-0.67; A_{250/260 nm} 1.18-1.30
• tárolási hőmérséklet: −20°C

Leírás

Alkalmazás

Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).

Tulajdonságok és előnyök

Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence

Minőség

Typical analysis:
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Szekvencia

Chain Length approx. 20,000 bp

In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.

As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.

Egység definíció

1 A₂₆₀ unit corresponds to 50 μg ds DNA.

Unit Conversion: 1 A₂₆₀ unit corresponds to 50 μg ds DNA.

Elkészítési megjegyzés

Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable