RAW 264.7 Cell Line murine

91062702, mouse blood, Macrophage

• Szinonimák: RAW264 7 Cells, RAW264.7 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• product name: RAW 264.7 Cell Line murine, Macrophage from blood, 91062702
• biológiai forrás: mouse blood
• növekedési mód: Semi-adherent
• kariotípus: Not specified
• morfológia: Macrophage
• termékek: Lysozyme
• receptorok: Immunoglobulin, complement
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Mouse monocyte macrophage

Sejtvonal leírása

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

Alkalmazás

Metabolic studies

RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.

Táptalaj

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

Egyéb megjegyzések

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