3T3 clone A31 Cell Line from mouse

86110401, mouse embryo, Fibroblast

• Szinonimák: 3T3-A31 Cells, BALB/3T3 clone A31 Cells, BALB/c 3T3 clone A31 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• Terméknév: 3T3 clone A31 Cell Line from mouse, 86110401
• biológiai forrás: mouse embryo
• leírás: Mouse BALB/c embryo
• növekedési mód: Adherent
• kariotípus: 2n = 40, hypotetraploid
• morfológia: Fibroblast
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Mouse BALB/c embryo

Sejtvonal leírása

Cells have now lost their contact inhibition.

Alkalmazás

3T3 clone A31 cell line from mouse has been used:

• to study in vitro cytotoxicity and genotoxicity of retinyl palmitate–loaded poly(lactide-co-glycolide) nanoparticles for the topical treatment of skin diseases
• for the characterization of the phototoxicity of Vemurafenib, which is a B-Raf serine/threonine kinase inhibitor
• for the phototoxicity assessment of systemically applied compounds and drug candidates

Tumourigenicity and virus studies: SV40 and MSV

Táptalaj

DMEM + 2mM Glutamine + 5% New Born Calf Serum (NBCS) + 5% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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