3T3-F442A
70654, mouse adipose tissue, Fibroblast-like
Szinonimák:
3T3F442A Cells, F442A Cells
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41106514
Javasolt termékek
product name
3T3-F442A, 00070654
biológiai forrás
mouse adipose tissue
növekedési mód
Adherent
kariotípus
Not specified
morfológia
Fibroblast-like
termékek
Not specified
receptorok
Not specified
technika/technikák
cell culture | mammalian: suitable
kiszállítva
dry ice
tárolási hőmérséklet
−196°C
Sejtvonal eredete
Mouse pre-adipocytes
Sejtvonal leírása
Cells will differentiate into adipocytes once confluent which takes approximately 10 days. Once confluent, cells should be grown in DMEM and 10% FCS and 5 micrograms/ml insulin. Media changes should take place every 48 h.
To manage customer expectations regarding the potential of 3T3 cell line stocks to differentiate into adipocytes, if using the cells for adipocyte differentiation please note: when cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g., 2 - 5 weeks, and the proportion of the population that differentiates can be limited. If 3T3 cells from an alternate source were previously used, we cannot guarantee the differentiation performance will be the same.We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.
To manage customer expectations regarding the potential of 3T3 cell line stocks to differentiate into adipocytes, if using the cells for adipocyte differentiation please note: when cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g., 2 - 5 weeks, and the proportion of the population that differentiates can be limited. If 3T3 cells from an alternate source were previously used, we cannot guarantee the differentiation performance will be the same.We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.
Alkalmazás
Once terminally differentiated the cells can be used as a model for either adipocyte differentiation or mature adipocytes.
Táptalaj
Szubkultúra rutin
Split sub-confluent cultures (70-80%) 1:3-1:5 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA, 5% CO2, 37°C. If cells are allowed to become confluent they will differentiate into adipocytes. If cryopreserving these cells, use New
Egyéb megjegyzések
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Analitikai tanúsítványok (COA)
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