SP2/O-Ag14 Cell Line murine

Non-producing hybridoma, 85072401

• Szinonimák: Mouse Cell Line
• UNSPSC kód: 12352207

Tulajdonságok

• biológiai forrás: mouse spleen
• növekedési mód: Suspension
• kariotípus: Not specified
• morfológia: Not specified
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Mouse x Mouse myeloma, non-producing

Sejtvonal leírása

Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. HAT sensitive mouse myeloma hybrid suitable for use as a hybridoma fusion partner - in this sense, functionally it is a myeloma cell line and often referred to as such in the literature.

Alkalmazás

Fusion partner

Táptalaj

RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. HAT sensitive.

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