MDCK Cell Line canine

84121903, canine kidney, Epithelial

• Szinonimák: MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• UNSPSC kód: 41106514
• eCl@ss: 32190102

Tulajdonságok

• product name: MDCK Cell Line canine, 84121903
• biológiai forrás: canine kidney
• form: cell suspension
• növekedési mód: Adherent
• kariotípus: 2n = 78
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Canine Cocker Spaniel kidney

Sejtvonal leírása

From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.

Alkalmazás

Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al

Táptalaj

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Egyéb megjegyzések

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