C9903

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder, enterotoxin

• Szinonimák: CTB
• MDL-szám: MFCD02356522
• UNSPSC kód: 12352200
• PubChem Substance ID: 329775212
• NACRES: NA.77

Tulajdonságok

• Terméknév: Cholera Toxin B subunit, ≥95% (SDS-PAGE), lyophilized powder
• Minőségi szint: 300
• Teszt: ≥95% (SDS-PAGE)
• Forma: lyophilized powder
• molekulatömeg: ~12 kDa
• összetétel: Protein, ~5% Lowry
• szennyeződések: ≤0.5% Cholera toxin A subunit (SDS-PAGE)
• oldhatóság: H₂O: 10 mg/mL
• tárolási hőmérséklet: 2-8°C
• SMILES string: CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
• InChI: 1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
• Nemzetközi kémiai azonosító kulcs: YDXZSNHARVUYNM-UHFFFAOYSA-N

Leírás

Általános leírás

Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.

Alkalmazás

Cholera Toxin B subunit has been used:

• for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
• in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Biokémiai/fiziológiai hatások

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Tulajdonságok és előnyök

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Kiszerelés

Package size based on protein content

Fizikai forma

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.

Feloldás

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN₃ and 1 mM sodium EDTA.

Analízis megjegyzés

Activity measured by ELISA using ganglioside GM₁-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.

Biztonsági adatok

• Piktogramok: GHS07
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H312 + H332,H412
• Óvintézkedésre vonatkozó mondatok: P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312
• Veszélyességi osztályok: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable