C7354

Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Szinonimák: Anti-CALB, Anti-D-28K
• MDL-szám: MFCD00164553
• UNSPSC kód: 12352203
• NACRES: NA.44

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• form: buffered aqueous solution
• molekulatömeg: antigen 28 kDa
• faj reaktivitás: rat, chicken, human
• fejlettebb validálás: independent; Learn more about Antibody Enhanced Validation
• technika/technikák: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using sections of rat, human or chicken cerebellum sections; microarray: suitable; western blot: 1:1,000 using recombinant rat calbindin-D-28K
• UniProt elérési szám: P05937
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... CALB1(793) ; mouse ... Calb1(12307) ; rat ... Calb1(83839)

Leírás

Általános leírás

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28 kDa calcium binding protein, with broad tissue distribution. Itbelongs , to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial cells.

Immunogen

synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 185-199). This sequence is identical in the corresponding human, mouse and bovine calbindin-D-28K sequences and is highly conserved (single amino acid substitution) in chicken and frog calbindin-D-28K.

Alkalmazás

Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit has been used in:

• immunocytochemistry
• western blotting
• microarray
• immunohistochemistry

Biokémiai/fiziológiai hatások

Calbindin 1 (CALB) is a calcium-binding protein. The protein may play a role in development of Purkinje cells found in heterotopias and cerebellar dysgenesias. It also acts as a very important component of intracellular homeostasis in cerebellar neurons. The progressive decrease of calbindin content in the Purkinje cells undergoes degeneration and death during paraneoplastic changes in the cerebellum. Calbindin effects on Parkinson′s disease (PD) risk displays population specificity.

Calbindin-D-28K is an important neuroanatomical marker. Despite its broad tissue distribution, it exhibits a cell-type-specific expression pattern. It has been immunocytochemically localized in selected cells in hippocampus, where it is thought to buffer intracellular calcium, or transport intramembranous calcium.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Analízis megjegyzés

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min. at RT or 37 °C) improves immunohistochemical staining.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Figyelmeztető mondatok: H412
• Óvintézkedésre vonatkozó mondatok: P273
• Veszélyességi osztályok: Aquatic Chronic 3
• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable