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C2978

Sigma-Aldrich

CelLytic™ M

Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

Szinonimák:

Whole-cell protein extraction solution

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About This Item

UNSPSC kód:
41116134
NACRES:
NA.56

Minőségi szint

200

form

solution

tárolási hőmérséklet

room temp

Általános leírás

CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.

Tulajdonságok és előnyök

  • Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
  • Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
  • Convenient: Ready-to-use reagent requires no scraping from culture plates
  • Fast: Rapid cell lysis at room temperature.

Mennyiség

CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).

Analízis megjegyzés

Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.

Jogi információk

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

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