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Merck

A6531

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat

affinity isolated antibody, suspension

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352203
NACRES:
NA.46

biológiai forrás

goat

Minőségi szint

konjugátum

agarose conjugate

antitest forma

affinity isolated antibody

antitest terméktípus

secondary antibodies

klón

polyclonal

form

suspension

faj reaktivitás

mouse

a címkézés mértéke

≥5 mg per mL

technika/technikák

Ouchterlony double diffusion: suitable
immunoprecipitation (IP): suitable

matrix

Cross-linked 4% beaded agarose

mátrix aktiválás

cyanogen bromide

mátrix távtartó

1 atom

kapacitás

0.4 mg/mL, resin binding capacity (mouse IgG)

tárolási hőmérséklet

2-8°C

célzott transzláció utáni módosítás

unmodified

Általános leírás

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies bind to all mouse IgG subclasses.

Immunogen

IgG isolated from pooled normal mouse serum

Alkalmazás

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies have been used for immunoprecipiation assays. The product can also be used for Ouchterlony double diffusion applications.
Soluble antigens from meningococci extracts were mixed with ascitic fluid containing primary antibody and 50 μl of agarose-linked goat anti-mouse IgG for 5 hours at 4 °C.

Biokémiai/fiziológiai hatások

IgG helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG2a of IgG has cytotropic properties and IgG2 molecules have the ability to activate complement.

Fizikai forma

Suspension in 0.5 M NaCl containing preservative

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

Riva Kovjazin et al.
PloS one, 9(1), e85400-e85400 (2014-01-15)
The MUC1 tumor associated antigen is highly expressed on a range of tumors. Its broad distribution on primary tumors and metastases renders it an attractive target for immunotherapy. After synthesis MUC1 is cleaved, yielding a large soluble extracellular alpha subunit
C R Tinsley et al.
Journal of general microbiology, 138(11), 2321-2328 (1992-11-01)
The neisserial Lip antigen is a conserved antigen associated with the pathogenic Neisseria species, and is composed of multiple repeats of a consensus pentapeptide. A series of monoclonal antibodies reacting with meningococcal Lip antigen were subjected to epitope mapping, using
Chakravarthi Chintalapati et al.
Molecular pharmacology, 90(5), 508-521 (2016-08-25)
Na+-d-glucose cotransporter 1 (SGLT1) is rate-limiting for glucose absorption in the small intestine. Shortly after intake of glucose-rich food, SGLT1 abundance in the luminal membrane of the small intestine is increased. This upregulation occurs via glucose-induced acceleration of the release
Ming-Shuo Chen et al.
Glia, 64(12), 2247-2262 (2016-10-21)
Axo-glial interactions are critical for myelination and the domain organization of myelinated fibers. Cell adhesion molecules belonging to the Cadm family, and in particular Cadm3 (axonal) and its heterophilic binding partner Cadm4 (Schwann cell), mediate these interactions along the internode.
Alvaro Román-Fernández et al.
Science advances, 9(5), eabq1858-eabq1858 (2023-02-04)
The glycocalyx component and sialomucin podocalyxin (PODXL) is required for normal tissue development by promoting apical membranes to form between cells, triggering lumen formation. Elevated PODXL expression is also associated with metastasis and poor clinical outcome in multiple tumor types.

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