Összes fotó(2)
Fontos dokumentumok
A6066
Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Szinonimák:
Rabbit Anti-Rat IgG (whole molecule)–AP
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
konjugátum
alkaline phosphatase conjugate
antitest forma
affinity isolated antibody
antitest terméktípus
secondary antibodies
klón
polyclonal
Forma
buffered aqueous glycerol solution
technika/technikák
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
western blot: 1:30,000-1:60,000
kiszállítva
wet ice
tárolási hőmérséklet
2-8°C
célzott transzláció utáni módosítás
unmodified
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Related Categories
Általános leírás
An immunoglobulin has two heavy chains and two lights chain connected by disulfide bond. It mainly helps in immune defense. IgG is a glycoprotein and is the major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
The affinity isolated antibody is obtained from anti-rat IgG antiserum by immunospecific purification, which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to rat IgG. Anti-Rat IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation.
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation.
Egyediség
Binds all rat Igs. Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rat immunoglobulins.
Immunogén
Purified rat IgG
Alkalmazás
Anti-Rat IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in:
- western blot analysis
- indirect enzyme-linked immunosorbent assay (ELISA)
- RNA immunoprecipitation assay
Biokémiai/fiziológiai hatások
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Fizikai forma
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Analitikai tanúsítványok (COA)
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