Összes fotó(1)
Fontos dokumentumok
A4541
Anti-Biotin−Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Szinonimák:
Anti-Biotin, Anti Biotin Hrp
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
NACRES:
NA.46
konjugátum:
peroxidase conjugate
application:
ELISA (d)
klón:
polyclonal
technika/technikák:
direct ELISA: 1:2,000
citations:
10
Javasolt termékek
biológiai forrás
goat
Minőségi szint
konjugátum
peroxidase conjugate
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
Forma
buffered aqueous solution
technika/technikák
direct ELISA: 1:2,000
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
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Általános leírás
Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. Goat anti-biotin-peroxidase antibody can be used in many applications where biotin may be introduced as target label.
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.
Immunogén
Biotin-KLH conjugate
Alkalmazás
Biotin-labeled proteins in U937 and BHK cell lysates were analyzed by western blot using HRP-conjugated goat anti-biotin.
Goat anti-biotin-peroxidase antibody can be used for direct ELISA assays at a dilution of 1:2,000.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Fizikai forma
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.
Elkészítési megjegyzés
Prepared by the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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