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P0044

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 3

DMSO solution, for the inhibition of protein phosphatase 2A, alkaline phosphatases, protein phosphatases 1 and 2A, DMSO solution

Szinonimák:

Phosphatase inhibitor

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

EC-szám:
200-664-3
UNSPSC kód:
12352200
NACRES:
NA.77

product name

Phosphatase Inhibitor Cocktail 3, DMSO solution

form

DMSO solution

Minőségi szint

200

kiszállítva

wet ice

tárolási hőmérséklet

2-8°C

Related Categories

Alkalmazás

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been tested on extract from animal tissues including human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells. It has been optimized and tested for L-isozymes of alkaline phosphatase as measured with pNPP and for serine-threonine protein phosphatases.

Biokémiai/fiziológiai hatások

This mixture contains individual components with specific inhibitory properties. Cantharidin inhibits protein phosphatase 2A. (-)-p-Bromolevamisole oxalate inhibits L-isoforms of alkaline phosphatases. Calyculin A inhibits protein phosphatases 1 and 2A.

Vigyázat

As supplied, this product is stable for four years at the recommended 2-8°C storage temperature.

Elkészítési megjegyzés

This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

kapcsolódó termék

Product No.
Leírás
Árazás

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1

Lobbanási pont (F)

188.6 °F

Lobbanási pont (C)

87 °C

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Jinglin L Xie et al.
Nature communications, 8(1), 499-499 (2017-09-13)
The capacity to coordinate environmental sensing with initiation of cellular responses underpins microbial survival and is crucial for virulence and stress responses in microbial pathogens. Here we define circuitry that enables the fungal pathogen Candida albicans to couple cell cycle
Tetsuro Ikegami et al.
PLoS pathogens, 5(2), e1000287-e1000287 (2009-02-07)
Rift Valley fever virus (RVFV) (genus Phlebovirus, family Bunyaviridae) is a negative-stranded RNA virus with a tripartite genome. RVFV is transmitted by mosquitoes and causes fever and severe hemorrhagic illness among humans, and fever and high rates of abortions in
Shumei Ma et al.
PloS one, 12(8), e0182921-e0182921 (2017-08-23)
Ferroptosis is a cell death pathway characterized by iron-dependent accumulation of reactive oxygen species (ROS) within the cell. The combination of siramesine, a lysosome disruptor, and lapatinib, a dual tyrosine kinase inhibitor, has been shown to synergistically induce cell death
Sarahmi Ishida et al.
The Plant cell, 20(12), 3273-3288 (2008-12-25)
The homeostasis of gibberellins (GAs) is maintained by negative feedback in plants. REPRESSION OF SHOOT GROWTH (RSG) is a tobacco (Nicotiana tabacum) transcriptional activator that has been suggested to play a role in GA feedback by the regulation of GA
Miao Zhao et al.
Oncotarget, 8(44), 77061-77074 (2017-11-05)
p21-activated kinase 4 (PAK4) regulates cell proliferation, apoptosis, cell motility and F-actin remodeling, but the PAK4 interactome has not been systematically analyzed. Here, we comprehensively characterized the human PAK4 interactome by iTRAQ quantitative mass spectrometry of PAK4-immunoprecipitations. Consistent with its
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A foszfatáz-inhibitorok használata kritikus fontosságú a foszforilációs vizsgálatok minden típusánál. Hasonlítsa össze a foszfatáz inhibitor koktélokat, hogy kiválaszthassa a kutatásaihoz legmegfelelőbbet.

Phosphatase Inhibitor Cocktail

The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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