63069

Magnesium chloride solution

BioUltra, for molecular biology, ~1 M in H₂O

• Szinonimák: Magnesium Chloride hydrate, Magnesium chloride (MgCl₂)hexahydrate, MgCl₂
• Lineáris képlet: MgCl₂
• CAS-szám: 7786-30-3
• Molekulatömeg: 95.21
• MDL-szám: MFCD00011106
• UNSPSC kód: 12352302
• PubChem Substance ID: 329759772
• NACRES: NA.26
• grade: for molecular biology
• Forma: liquid
• oldhatóság: water: soluble

Tulajdonságok

• grade: for molecular biology
• Minőségi szint: 200
• termékcsalád: BioUltra
• Forma: liquid
• koncentráció: ~1 M in H₂O
• technika/technikák: HPLC: suitable; flow cytometry: suitable
• szennyeződések: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.0002% total Nitrogen (N)
• pH: 5.0-7.5 (25 °C, 1 M in H₂O)
• oldhatóság: water: soluble
• sűrűség: 1.08 g/mL at 20 °C
• anion nyomok: phosphate (PO₄^3-): ≤10 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• kation nyomok: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤10 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤50 mg/kg; Li: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤500 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: 1 M in H₂O
• UV-elnyelés: λ: 260 nm A_max: 0.05; λ: 280 nm A_max: 0.03
• alkalmasság: suitable for molecular biology
• SMILES string: Cl[Mg]Cl
• alkalmazás(ok): cell analysis; filtration; vaccine development
• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2
• Nemzetközi kémiai azonosító kulcs: TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Leírás

Általános leírás

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg²⁺ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg²⁺ enhance the stability of primer-template duplexes and the efficiency of amplification.

Alkalmazás

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).

Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).

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Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves