DNTPM-RO

PCR Nucleotide Mix

solution, sufficient for 500 reactions (11581295001), sufficient for 5,000 reactions (11814362001), sufficient for 2,500 reactions (04638956001)

• UNSPSC kód: 41106300
• NACRES: NA.21

Tulajdonságok

• grade: Molecular Biology
• Minőségi szint: 100
• Teszt: 99.8% (sum of 4 peaks, HPLC)
• Forma: solution
• használat: sufficient for 2,500 reactions (04638956001); sufficient for 5,000 reactions (11814362001); sufficient for 500 reactions (11581295001)
• kiszerelés: pkg of 10 × 200 μL (11814362001 [10mM, each]); pkg of 200 μL (11581295001 [10 mM, each]); pkg of 5 × 200 μL (04638956001 [10 mM, each])
• gyártó/kereskedő neve: Roche
• koncentráció: 10 mM
• szennyeződések: DNase, none detected; RNase, none detected
• szín: colorless
• oldhatóság: water: miscible
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.

Alkalmazás

• The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions: PCR
• RT-PCR
• cDNA synthesis
• Primer extension

Tulajdonságok és előnyök

The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.

Improve yield and performance

• Choose a convenient ready-to-use mix of all 4 nucleotides.
• Achieve the highest PCR and RT-PCR sensitivity.
• Insist on highest purity (>99%) nucleotides.

Kiszerelés

1 set containing 4 ready-to-use mixes

Minőség

Function Testing in PCR
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.

Elkészítési megjegyzés

Activator: Mg²⁺
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• dATP, PCR Grade, sodium salt 10 mM
• dCTP, PCR Grade, sodium salt 10 mM
• dGTP, PCR Grade, sodium salt 10 mM
• dTTP, PCR Grade, sodium salt 10 mM

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: nwg
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash