TAQ-RO

Taq DNA Polymerase, 5 U/μl

optimum pH ~9.0 (20 °C), optimum reaction temp. 72 °C

• Szinonimák: dna amplification, pcr, polymerase, pcr, primer extension, dna amplification, primer extension
• Enzyme Commission szám: 2.7.7.7 (BRENDA, IUBMB)
• UNSPSC kód: 41106300

Tulajdonságok

• biológiai forrás: bacterial (Thermus aquaticus)
• Minőségi szint: 100
• rekombináns: expressed in E. coli
• grade: Molecular Biology
• Forma: liquid
• használat: sufficient for ≤1,000 reactions (11146173001); sufficient for ≤10,000 reactions (11435094001); sufficient for ≤2,000 reactions (11418432001); sufficient for ≤5,000 reactions (11596594001); sufficient for ≤200 reactions (11146165001)
• specifikus aktivitás: 5 U/μL
• molekulatömeg: 95 kDa
• kiszerelés: pkg of 1,000 U (11418432001 [4 x 250 U]); pkg of 2,500 U (11596594001 [10 x 250 U]); pkg of 5,000 U (11435094001 [20 x 250 U]); pkg of 100 U (11146165001); pkg of 500 U (11146173001 [1 x 500 U])
• gyártó/kereskedő neve: Roche
• parameter: 72 °C optimum reaction temp.
• technika/technikák: DNA sequencing: suitable; PCR: suitable
• szín: colorless
• optimális pH: ~9.0 (20 °C)
• oldhatóság: water: miscible
• alkalmasság: suitable for molecular biology
• NCBI elérési szám: AAA27507
• UniProt elérési szám: P19821
• alkalmazás(ok): genomic analysis; life science and biopharma
• idegen aktivitás: Nicking activity 30 units, none detected; Ribonuclease 30 units, none detected; Unspecific endonucleases 30 units, none detected
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme was cloned in E.coli.

Alkalmazás

Taq DNA Polymerase can be used in simple, routine PCR. Roche Applied Science Taq DNA polymerase is held to rigorous purity and quality standards. This preparation of recombinant Taq DNA Polymerase can be applied for:

• PCR
• RT-PCR
• qPCR
• Other primer-extension reactions, such as sequencing and labeling

Tulajdonságok és előnyök

Reliable reproducible results:
High lot-to-lot consistency.
No need to test each lot:
Taq DNA Polymerase is rigorously tested.
Prevent PCR carryover:
dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.

Kiszerelés

1 kit containing 2 components

Minőség

Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.

Egység definíció

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 60 min at +65 °C under the assay conditions given above.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl₂, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α^-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 5 U/μl

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Jogi információk

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Only

• Taq DNA Polymerase 5 U/μl
• PCR Buffer with MgCl₂ 10x concentrated
• MgCl₂ Stock Solution
• PCR Buffer without MgCl₂

Biztonsági adatok

• Figyelmeztető mondatok: H412
• Óvintézkedésre vonatkozó mondatok: P273 - P501
• Veszélyességi osztályok: Aquatic Chronic 3
• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash