Összes fotó(1)
Fontos dokumentumok
11644793001
Roche
Cytotoxicity Detection Kit (LDH)
suitable for protein quantification, suitable for cell analysis, detection, sufficient for ≤2,000 tests
Szinonimák:
LDH
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352200
Javasolt termékek
használat
sufficient for ≤2,000 tests
Minőségi szint
kiszerelés
pkg of 1 kit
gyártó/kereskedő neve
Roche
technika/technikák
protein quantification: suitable
λmax
490 nm
alkalmazás(ok)
cell analysis
detection
észlelési módszer
colorimetric
tárolási hőmérséklet
−20°C
Általános leírás
A nonradioactive alternative to the [51Cr] and [3H]-thymidine release assay.
Colorimetric assay for the quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant.
Colorimetric assay for the quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant.
Alkalmazás
The Cytotoxicity Detection Kit (LDH) is a fast and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well plate format. Thus, the kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example:
- Detection and quantification of cell-mediated cytotoxicity
- Determination of mediator-induced cytolysis
- Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries
- Determination of cell death in bioreactors
- Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth
Kiszerelés
1 kit containing 2 components.
Elkészítési megjegyzés
Working solution: Vial 1 Catalyst:
Reconstitute the lyophilisate in 1 ml double dist. water for 10 minutes and mix thoroughly.
Vial 2 Dye solution:
Ready-to-use solution.
Reaction mixture
For 100 tests: Shortly before use, mix 250 μl of vial 1 with 11.25 ml of vial 2.
For 400 tests: Shortly before use, add the total volume of catalyst (1 ml) to the total volume
of dye solution (45 ml) and mix well.
Note: The Reaction mixture should not be stored; prepare immediately before use.
Storage conditions (working solution): 2 to 8 °C
The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution is stable for four weeks when stored at 2 to 8 °C.
Once thawed, the dye solution is stable for several weeks when stored at 2 to 8 °C.
Reconstitute the lyophilisate in 1 ml double dist. water for 10 minutes and mix thoroughly.
Vial 2 Dye solution:
Ready-to-use solution.
Reaction mixture
For 100 tests: Shortly before use, mix 250 μl of vial 1 with 11.25 ml of vial 2.
For 400 tests: Shortly before use, add the total volume of catalyst (1 ml) to the total volume
of dye solution (45 ml) and mix well.
Note: The Reaction mixture should not be stored; prepare immediately before use.
Storage conditions (working solution): 2 to 8 °C
The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution is stable for four weeks when stored at 2 to 8 °C.
Once thawed, the dye solution is stable for several weeks when stored at 2 to 8 °C.
Egyéb megjegyzések
Kit Components Only
Product No.
Leírás
- Catalyst (Diaphorase/NAD+ mixture)
- Dye Solution (INT and sodium lactate)
kapcsolódó termék
Product No.
Leírás
Árazás
Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Irrit. 2
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
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Cikkek
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
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