10102105001

Adenosine Deaminase (ADA)

from calf intestine

• Szinonimák: ADA
• Enzyme Commission szám: 3.5.4.4 (BRENDA, IUBMB)
• UNSPSC kód: 12352204

Tulajdonságok

• biológiai forrás: bovine (calf) intestine
• Minőségi szint: 100
• form: solution
• specifikus aktivitás: ~200 units/mg protein (At 25 °C with adenosine as the substrate.)
• molekulatömeg: 230-440 kDa (high); 30-47 kDa (low )
• kiszerelés: pkg of 2 mL (10 mg)
• gyártó/kereskedő neve: Roche
• optimális pH: 7.0-7.4
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.

Adenosine deaminase (ADA) is a zinc metalloenzyme.

Alkalmazás

Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.

Adenosine deaminase has been used to pre-treat primary Kupffer cells.

Biokémiai/fiziológiai hatások

Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Minőség

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Fizikai forma

Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6

Elkészítési megjegyzés

Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash