The validation data and assay characteristics are provided in the back of each assay protocol.
HSTCMAG-28SK
MILLIPLEX® Human High Sensitivity T Cell Panel - Immunology Multiplex Assay
Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Szinonimák:
Human high-sensitivity cytokine multiplex kit, Luminex® human high-sensitivity cytokine immunoassay, Millipore human high-sensitivity cytokine panel
About This Item
Javasolt termékek
Minőségi szint
faj reaktivitás
human
gyártó/kereskedő neve
Milliplex®
assay range
accuracy: 98-107%
sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC)
standard curve range: 0.18-750 pg/mL
(IL-6)
standard curve range: 0.24-1,000 pg/mL
(IL-13)
standard curve range: 0.24-1,000 pg/mL
(IL-21)
standard curve range: 0.31-1,250 pg/mL
(IL-8)
standard curve range: 0.31-1,250 pg/mL
(MIP-1α)
standard curve range: 0.37-1,500 pg/mL
(IL-7)
standard curve range: 0.45-1,750 pg/mL
(TNFα)
standard curve range: 0.49-2,000 pg/mL
(IL-1β)
standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70))
standard curve range: 0.49-2,000 pg/mL
(IL-2)
standard curve range: 0.49-2,000 pg/mL
(IL-5)
standard curve range: 0.61-2,500 pg/mL
(IFNγ)
standard curve range: 0.61-2,500 pg/mL
(MIP-3α)
standard curve range: 0.73-3,000 pg/mL
(IL-17A)
standard curve range: 0.92-3,750 pg/mL
(MIP-1β)
standard curve range: 1.22-5,000 pg/mL
(GM-CSF)
standard curve range: 1.46-6,000 pg/mL
(IL-10)
standard curve range: 1.46-6,000 pg/mL
(ITAC)
standard curve range: 1.83-7,500 pg/mL
(IL-4)
standard curve range: 18.3-75,000 pg/mL
(Fractalkine)
standard curve range: 7.93-32,500 pg/mL
(IL-23)
inter-assay cv: <15%
intra-assay cv: <10%
technika/technikák
multiplexing: suitable
észlelési módszer
fluorometric (Luminex xMAP)
kiszállítva
wet ice
Általános leírás
The MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel is to be used for the simultaneous quantification of any or all of the following analytes in human plasma, serum, and cell/tissue culture supernatant samples: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, ITAC, MIP-1α, MIP-1β, MIP-3α
and TNFα.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
Egyediség
There is no detectable cross-reactivity within the panel.
Alkalmazás
- Analytes: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, I-TAC, MIP-1α, MIP-1β, MIP-3α, TNF-α
- Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay.
- Recommended Sample type: Serum, plasma or tissue/cell lysate and culture supernatant
- Assay Run Time: Overnight
- Research Category: Inflammation & Immunology
- Research Subcategory: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease, Neuroscience
Tulajdonságok és előnyök
Kapcsolódás
Tárolás és stabilitás
Egyéb megjegyzések
Jogi információk
Jogi nyilatkozat
javasolt
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Célzott szervek
Respiratory Tract
Tárolási osztály kódja
10 - Combustible liquids
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Related Content
Immunology multiplex assays, like MILLIPLEX® assays, allow researchers to investigate more immune biomarkers simultaneously, saving time and resources. This is especially critical to immunology research, where numerous analytes play a role in different pathways.
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Could you provide me with more information about the T cell cytokine assay, including CV values and detection limits? The current information I have is that serum concentrations of 13 human cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p70, IL-13, GM-CSF, IFN-γ, and TNF-α) were quantified (pg/ml) in the serum of 52 matched cord blood and maternal venous blood samples using the human 13-plex magnetic bead assay (HCYTOMAG-60K). The assay protocol followed the manufacturer's guidelines, and standards and controls were included in each run. I also cc-ed Jennifer as she was involved in my work and may have previous information about technical reports.
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