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Merck

HCMP1MAG-19K

Millipore

MILLIPLEX® Human Complement Panel 1 - Immunology Multiplex Assay

The Human Complement Panel 1 Bead-Based Multiplex Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of complement proteins and factors in human serum, plasma and cell culture samples.

Szinonimák:

Human complement multiplex kit, Luminex® human complement immunoassay, Millipore human complement panel

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Az árazás és az elérhetőség jelenleg nem elérhető

Minőségi szint

faj reaktivitás

human

gyártó/kereskedő neve

Milliplex®

assay range

accuracy: 96%
(Mannose-Binding Lectin (MBL))

sensitivity: 0.0051 ng/mL
(Complement C5a)

sensitivity: 0.026 ng/mL
(Adipsin (Complement Factor D))

sensitivity: 0.040 ng/mL
(Mannose-Binding Lectin (MBL))

sensitivity: 0.22 ng/mL
(Complement Factor I)

sensitivity: 0.31 ng/mL
(Complement C2)

sensitivity: 0.36 ng/mL
(Complement C4b)

sensitivity: 1.040 ng/mL
(Complement C5)

standard curve range: 0.069-50 ng/mL
(Adipson/ Complement Factor D)

standard curve range: 0.07-50 ng/mL
(Complement C2)

standard curve range: 0.137-100 ng/mL
(Mannose-Binding Lectin (MBL))

standard curve range: 0.69-500 ng/mL
(Complement Factor I)

standard curve range: 1.37-1,000 ng/mL
(Complement C4b)

standard curve range: 2.74-2,000 ng/mL
(Complement C5)

standard curve range: 4.12-30,000 pg/mL
(Complement C5a)

technika/technikák

multiplexing: suitable

észlelési módszer

fluorometric (Luminex xMAP)

kiszállítva

2-8°C

Általános leírás

The complement system consists of many plasma proteins that assist the ability of phagocytic cells and antibodies to clear pathogens. There are three different simultaneous pathways. The classical pathway is stimulated by antigen-antibody complexes; the alternative pathway spontaneously activates on contact with pathogenic cell surfaces; and the mannose-binding lectin (MBL) pathway recognizes mannose sugars usually present only on pathogenic cell surfaces. Because of the potential to be extremely damaging to host tissues, complement system activation must be tightly controlled. The regulating complement control proteins, including CD59, also known as protectin, are present at a higher concentration in the blood plasma than the complement proteins themselves. The complement system is thought to play a key role in many diseases with an immune component, such as asthma and many autoimmune diseases, including SLE, IBD and multiple sclerosis. It is also becoming increasingly associated with neurological disease, such as Alzheimer′s disease and conditions such as spinal cord injuries.

MILLIPLEX® Complement Panel 1 Bead Panel is an 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: C2, C4B, C5, C5a, Adipsin/Complement Factor D, MBL and Complement Factor I.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Egyediség

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Alkalmazás

  • Analytes: C2, C4b, C5, C5a, Adipsin/Factor D, Mannose-Binding Lectin (MBL), and Factor I
  • Recommended Sample type: Serum, plasma, and tissue/cell culture supernatant
  • Recommended Sample dilution: 1:200 diluted plasma or serum. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
  • Assay Run Time: Overnight or two-hour primary incubation
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Inflammation
  • Note: To avoid complement activation in samples, which will affect the results, samples should be thawed as rapidly as possible, put on ice and assayed immediately

Tulajdonságok és előnyök

Design your multiplex kit by choosing available analytes within this panel.

Kiszerelés

96 well plate

Tárolás és stabilitás

Recommended storage for kit components is 2 - 8°C.

Jogi információk

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Figyelmeztetés

Danger

Veszélyességi osztályok

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Célzott szervek

Respiratory Tract

Tárolási osztály kódja

10 - Combustible liquids


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Frank Sebastian Fröschen et al.
Diagnostics (Basel, Switzerland), 11(3) (2021-04-04)
The role and diagnostic value of the synovial complement system in patients with low-grade periprosthetic joint infection (PJI) are unclear. We sought to evaluate, for the first time, the usefulness of synovial complement factors in these patients by measuring the
Mikyle David et al.
Archives of gynecology and obstetrics, 304(6), 1467-1473 (2021-04-22)
In preeclampsia, there are excessive complement components expressed due to increased complement activation; therefore, this study investigated the concentration of adipsin and C9 in HIV-associated preeclampsia. The study population (n = 76) was stratified by pregnancy type (normotensive pregnant and preeclampsia) and
Ngo Truong Giang et al.
Scientific reports, 10(1), 14923-14923 (2020-09-12)
The complement system may be crucial during dengue virus infection and progression to severe dengue. This study investigates the role of MBL2 genetic variants and levels of MBL in serum and complement proteins in Vietnamese dengue patients. MBL2 genotypes (- 550L/H
Pavan Bhargava et al.
Multiple sclerosis (Houndmills, Basingstoke, England), 27(4), 509-518 (2020-07-17)
Synaptic loss is a feature of multiple sclerosis pathology that can be seen even in normal-appearing gray matter. Opsonization of synapses with complement components may underlie pathologic synapse loss. We sought to determine whether circulating neuronal-enriched and astrocytic-enriched extracellular vesicles
Manjunath Ramanjaneya et al.
Frontiers in endocrinology, 12, 641361-641361 (2021-04-17)
Gestational Diabetes Mellitus (GDM) development is related to underlying metabolic syndrome that is associated with elevated complement C3 and C4. Elevated C3 levels have been associated with preeclampsia and the development of macrosomia. This case-control study included 34 pregnant women

Questions

1–5 of 5 Questions  
  1. Does the C5a on the Milliplex Complement panel 1 detect the total or free C5a?

    1 answer
    1. We have not tested the assay for free C5a only. The antibody specificity information does not list this detail. The immunogen is only listed as purified human C5a.

      Helpful?

  2. Can the Human Milliplex Complement Panels HCMP1MAG-19K and HCMP2MAG-19K be used to measure Complement Factors in rabbits?

    1 answer
    1. The Human Complement Milliplex panels have not been tested with rabbit samples. There are no references available where this has been done. It is uncertain whether the kits can detect rabbit samples.

      Helpful?

  3. Do the Human Complement Milliplex Panels cross-react with mice?

    1 answer
    1. In our testing, 8 mouse samples were tested in both Human Complement Panels, and only Factor B was detected. While we cannot definitively assert that there is no cross-reactivity, it is worth noting that the lack of signal could be attributed to low levels in the few samples tested. However, we did not observe any obvious cross-reactivity.

      Helpful?

  4. Do the Adipsin antibodies exhibit consistency between the Human Adipokine Panel 1 (HADK1MAG-61K) and Human Complement Panel 1 (HCMP1MAG-19K)? Is there a correlation in the sample data?

    1 answer
    1. Certainly, the Adipsin capture and detection antibodies are consistent across both panels. Furthermore, the sample data demonstrates a high correlation, with an R value of 0.99 and a slope of 0.7.

      Helpful?

  5. Any other instruments can be used to detect the signal?

    1 answer
    1. Unfortunately, the use of this product is currently limited to the Luminex instrument.

      Helpful?

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