We have not tested the assay for free C5a only. The antibody specificity information does not list this detail. The immunogen is only listed as purified human C5a.
HCMP1MAG-19K
MILLIPLEX® Human Complement Panel 1 - Immunology Multiplex Assay
The Human Complement Panel 1 Bead-Based Multiplex Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of complement proteins and factors in human serum, plasma and cell culture samples.
Szinonimák:
Human complement multiplex kit, Luminex® human complement immunoassay, Millipore human complement panel
About This Item
Javasolt termékek
Minőségi szint
faj reaktivitás
human
gyártó/kereskedő neve
Milliplex®
assay range
accuracy: 96%
(Mannose-Binding Lectin (MBL))
sensitivity: 0.0051 ng/mL
(Complement C5a)
sensitivity: 0.026 ng/mL
(Adipsin (Complement Factor D))
sensitivity: 0.040 ng/mL
(Mannose-Binding Lectin (MBL))
sensitivity: 0.22 ng/mL
(Complement Factor I)
sensitivity: 0.31 ng/mL
(Complement C2)
sensitivity: 0.36 ng/mL
(Complement C4b)
sensitivity: 1.040 ng/mL
(Complement C5)
standard curve range: 0.069-50 ng/mL
(Adipson/ Complement Factor D)
standard curve range: 0.07-50 ng/mL
(Complement C2)
standard curve range: 0.137-100 ng/mL
(Mannose-Binding Lectin (MBL))
standard curve range: 0.69-500 ng/mL
(Complement Factor I)
standard curve range: 1.37-1,000 ng/mL
(Complement C4b)
standard curve range: 2.74-2,000 ng/mL
(Complement C5)
standard curve range: 4.12-30,000 pg/mL
(Complement C5a)
technika/technikák
multiplexing: suitable
észlelési módszer
fluorometric (Luminex xMAP)
kiszállítva
2-8°C
Általános leírás
MILLIPLEX® Complement Panel 1 Bead Panel is an 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: C2, C4B, C5, C5a, Adipsin/Complement Factor D, MBL and Complement Factor I.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Immune Response
Egyediség
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Alkalmazás
- Analytes: C2, C4b, C5, C5a, Adipsin/Factor D, Mannose-Binding Lectin (MBL), and Factor I
- Recommended Sample type: Serum, plasma, and tissue/cell culture supernatant
- Recommended Sample dilution: 1:200 diluted plasma or serum. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
- Assay Run Time: Overnight or two-hour primary incubation
- Research Category: Inflammation & Immunology
- Research Subcategory: Inflammation
- Note: To avoid complement activation in samples, which will affect the results, samples should be thawed as rapidly as possible, put on ice and assayed immediately
Tulajdonságok és előnyök
Kiszerelés
Tárolás és stabilitás
Jogi információk
Jogi nyilatkozat
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Célzott szervek
Respiratory Tract
Tárolási osztály kódja
10 - Combustible liquids
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
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Does the C5a on the Milliplex Complement panel 1 detect the total or free C5a?
1 answer-
Helpful?
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Can the Human Milliplex Complement Panels HCMP1MAG-19K and HCMP2MAG-19K be used to measure Complement Factors in rabbits?
1 answer-
The Human Complement Milliplex panels have not been tested with rabbit samples. There are no references available where this has been done. It is uncertain whether the kits can detect rabbit samples.
Helpful?
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Do the Human Complement Milliplex Panels cross-react with mice?
1 answer-
In our testing, 8 mouse samples were tested in both Human Complement Panels, and only Factor B was detected. While we cannot definitively assert that there is no cross-reactivity, it is worth noting that the lack of signal could be attributed to low levels in the few samples tested. However, we did not observe any obvious cross-reactivity.
Helpful?
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Do the Adipsin antibodies exhibit consistency between the Human Adipokine Panel 1 (HADK1MAG-61K) and Human Complement Panel 1 (HCMP1MAG-19K)? Is there a correlation in the sample data?
1 answer-
Certainly, the Adipsin capture and detection antibodies are consistent across both panels. Furthermore, the sample data demonstrates a high correlation, with an R value of 0.99 and a slope of 0.7.
Helpful?
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Any other instruments can be used to detect the signal?
1 answer-
Unfortunately, the use of this product is currently limited to the Luminex instrument.
Helpful?
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