Ugrás a tartalomra
Merck
Összes fotó(2)

Fontos dokumentumok

ABS234

Sigma-Aldrich

Anti-PI3 Kinase Antibody, p85

from rabbit, purified by affinity chromatography

Szinonimák:

Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-al

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
IP
WB
faj reaktivitás:
rat, human
technika/technikák:
immunoprecipitation (IP): suitable
western blot: suitable
citations:
11

biológiai forrás

rabbit

Minőségi szint

100

antitest forma

affinity isolated antibody

antitest terméktípus

primary antibodies

klón

polyclonal

tisztítva

affinity chromatography

faj reaktivitás

rat, human

faj reaktivitás (homológia által előrejelzett)

mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)

technika/technikák

immunoprecipitation (IP): suitable
western blot: suitable

NCBI elérési szám

NP_037137

UniProt elérési szám

Q63787

kiszállítva

wet ice

célzott transzláció utáni módosítás

unmodified

Géninformáció

bovine ... Pik3R1(282307)
human ... PIK3R1(5295)
mouse ... Pik3R1(18708)
rat ... Pik3R1(25513)
rhesus monkey ... Pik3R1(698996)

Általános leírás

Phosphatidylinositol 3-Kinase (PI3 Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β,γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibited by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

Immunogén

GST-tagged recombinant protein corresponding to rat PI3 Kinase, p85.

Alkalmazás

Detect PI3 Kinase, p85 using this rabbit polyclonal antibody, Anti-PI3 Kinase Antibody, p85 validated for use in western blotting & IP.
Immunoprecipitation Analysis: A representative lot immunoprecipitated PI3 Kinase, p85 in 0.5 mg of Jurkat cell lysate.

Minőség

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:600 dilution of this antibody detected PI3 Kinase, p85 in 10 µg of Jurkat cell lysate.

Cél megnevezése

~85 kDa observed

Kapcsolódás

Replaces: 06-497

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Bruna Gazzi de Lima-Seolin et al.
Oxidative medicine and cellular longevity, 2019, 6325424-6325424 (2019-07-31)
The increased circulation of norepinephrine, found in the diseased heart as a result of sympathetic nervous system overactivation, is responsible for its cardiotoxic effects including pathological hypertrophy, cell death, and oxidative stress. Bucindolol is a third generation adrenergic blocker, which
Flemming Dela et al.
Acta physiologica (Oxford, England), 226(2), e13245-e13245 (2018-12-27)
To examine the effect of high-intensity interval training (HIIT) on glucose clearance rates in skeletal muscle and explore the mechanism within the muscle. Ten males with type 2 diabetes mellitus (T2DM) and ten matched healthy subjects performed 2 weeks of one-legged
Anastasiia Stratiievska et al.
eLife, 7 (2018-12-19)
Although it has been known for over a decade that the inflammatory mediator NGF sensitizes pain-receptor neurons through increased trafficking of TRPV1 channels to the plasma membrane, the mechanism by which this occurs remains mysterious. NGF activates phosphoinositide 3-kinase (PI3K)
Scott E LeBlanc et al.
Nucleic acids research, 44(11), 5133-5147 (2016-03-05)
PPARγ2 is a critical lineage-determining transcription factor that is essential for adipogenic differentiation. Here we report characterization of the three-dimensional structure of the PPARγ2 locus after the onset of adipogenic differentiation and the mechanisms by which it forms. We identified
Mathews Valuparampil Varghese et al.
American journal of physiology. Lung cellular and molecular physiology, 320(4), L508-L521 (2021-01-28)
We have previously reported that several patients with idiopathic pulmonary hypertension (PH) had different types of G6PD deficiency. However, the role of G6PD in PH is multifactorial because G6PD is involved in controlling oxidative stress, metabolic switch, and red blood
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