ABN182
Anti-CYP27B1 Antibody
from rabbit, purified by affinity chromatography
Szinonimák:
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytoch
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
mouse, rat, human
faj reaktivitás (homológia által előrejelzett)
chimpanzee (based on 100% sequence homology)
technika/technikák
immunohistochemistry: suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... CYP27B1(1594)
Általános leírás
25-hydroxyvitamin D-1 alpha hydroxylase (1αOHase; VD3 1A hydroxylase; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; or CYP27B1) is a mitochondrial enzyme that belongs to the cytochrome P450 family. It catalyzes the hydroxylation of 25-hydroxyvitamin D3 to form an active 1-alpha, 25-dihydroxyvitamin D3, in the kidneys and other tissues. 25-hydroxyvitamin D-1 alpha hydroxylase plays an important role in bone growth, calcium metabolism, and tissue differentiation. Previous studies have reported that mice deficient in 25-hydroxyvitamin D-1 alpha hydroxylase are affected by hypocalcemia, secondary hyperparathyroidism, retarded growth, and the skeletal abnormalities. In humans, defects in this enzyme causes pseudovitamin D deficiency rickets (vitamin D-dependent type 1A rickets), an autosomal recessive condition characterized by abnormal bone mineralization and impaired growth.
Immunogen
KLH-conjugated linear peptide corresponding to human CYP27B1.
Alkalmazás
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CYP27B1 in mouse and rat hippocampus tissue and in rat kidney tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
This Anti-CYP27B1 Antibody is validated for use in Western Blotting, IHC for the detection of CYP27B1.
Minőség
Evaluated by Western Blot in serum free MCF-7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected CYP27B1 in 10 µg of serum free MCF-7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected CYP27B1 in 10 µg of serum free MCF-7 cell lysate.
Cél megnevezése
~58 kDa observed. Uncharacterized bands may be observed at ~55 kDa, ~70 kDa, ~110 kDa, and ~115 kDa in some cell lysates. A doublet at ~58 kDa may be observed in some cell lysates (Kemmis, C. M., et al. (2006). J Nutr. 136(4):887-892.).
Fizikai forma
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Tárolás és stabilitás
Stable for 1 year at 2-8°C from date of receipt.
Analízis megjegyzés
Control
Serum free MCF-7 cell lysate
Serum free MCF-7 cell lysate
Egyéb megjegyzések
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Xiaochen Li et al.
Frontiers in pharmacology, 12, 690181-690181 (2021-06-22)
Osteoarthritis (OA) is a worldwide degenerative joint disease that seriously impaired the quality of life of patients. OA has been established as a disease with metabolic disorder. Cholesterol 25-hydroxylase (CH25H) was proved to play a key role in cartilage cholesterol
Yi Jiang et al.
International journal of endocrinology, 2020, 7257913-7257913 (2020-05-01)
Parathyroid glands contain the vitamin D receptor (VDR) and 25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24A1), which catalyze the production and degradation of 1,25-dihydroxyvitamin D [1,25(OH)2D], respectively. Previous studies have shown that the serum level of intact parathyroid hormone (iPTH) increases
Eva S Liu et al.
Endocrinology, 155(10), 3750-3756 (2014-07-25)
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