ABN1647
Anti-Vesicular Glutamate Transporter 1 Antibody
rabbit polyclonal
Szinonimák:
Vesicular glutamate transporter 1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
IF
IHC
WB
immunoautoradiography
IHC
WB
immunoautoradiography
faj reaktivitás:
mouse, human, rat
technika/technikák:
immunoautoradiography: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
7
Javasolt termékek
Terméknév
Anti-VGluT1, from rabbit
biológiai forrás
rabbit
Minőségi szint
antitest forma
unpurified
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
mouse, human, rat
faj reaktivitás (homológia által előrejelzett)
bovine (based on 100% sequence homology)
kiszerelés
antibody small pack of 25 μL
technika/technikák
immunoautoradiography: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
izotípus
IgG
NCBI elérési szám
UniProt elérési szám
kiszállítva
ambient
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... SLC17A7(57030)
Általános leírás
Vesicular glutamate transporter 1 (UniProt: also known as VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7) is encoded by the Slc17a7 (also known as Bnpi, Vglut1) gene (Gene ID: 116638) in rat. VGLUT1 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neuronal cells. Glutamate transporters mediate glutamate uptake into synaptic vesicles and are driven by a proton electrochemical gradient generated by the vacuolar H+-ATPase. Two isoforms of VGLUT1 have been reported that are produced by alternative splicing. VGLUT1 is expressed in the cerebellum, cerebral cortex and hippocampus. Expression of isoform VGLUT2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGLUT1 and VGLUT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGLUT2 expression predominates. VGLUT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1. The VGLUT1 knockout mice die at the age when a strong upregulation of VGLUT1 through newly emerging VGLUT1 synapses as well as a replacement of the previously predominant VGLUT2 isoform in cerebellar, hippocampal, and cortical neurons occurs. (Ref.: Wojcik, SM et al. (2004). Proc. Natl. Acad. Sci. USA 101(18): 7158-7163).
Egyediség
This rabbit polyclonal antibody detects Vesicular glutamate transporter 1 in Human, Mouse, and Rat brain. It targets an epitope with in 14 amino acids from the C-terminal region.
Immunogén
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of rat Vesicular glutamate transporter 1 (VGLUT1).
Alkalmazás
Anti-VGLUT1, Cat. No. ABN1647, is a highly specific rabbit polyclonal antibody that targets Vesicular glutamate transporter 1 and has been tested in Immunoautoradiography, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunoautoradiography Analysis: A representative lot detected VGLUT1 in rat and mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).
Immunohistochemistry Analysis: A representative lot detected VGLUT1 in prefrontal cortex of a representative control subject and AD patient (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).
Western Blotting Analysis: A representative lot detected VGLUT1 in glutamate transporter 1 in rat and human putamen extracts (Kashani, A., et. al. (2007). Neurobiol Agining 28(4):568-78) and prefrontal cortex of controls, MCI, early stage Alzheimer (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).
Immunofluorescence Analysis: A representative lot detected VGLUT1 in mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).
Immunofluorescence Analysis: A representative lot detected VGLUT1 in rat striatum (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Immunoautoradiography Analysis: A representative lot detected VGLUT1 in coronal sections of frontal rat brain (Herzog, E., et. al. (2004). Neuroscience. 123(4):983-1002) and rat brain sagittal sections (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Western Blotting Analysis: A representative lot detected VGLUT1 in BON cells stably expressing VGLUT1 (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Immunohistochemistry Analysis: A representative lot detected VGLUT1 in prefrontal cortex of a representative control subject and AD patient (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).
Western Blotting Analysis: A representative lot detected VGLUT1 in glutamate transporter 1 in rat and human putamen extracts (Kashani, A., et. al. (2007). Neurobiol Agining 28(4):568-78) and prefrontal cortex of controls, MCI, early stage Alzheimer (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).
Immunofluorescence Analysis: A representative lot detected VGLUT1 in mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).
Immunofluorescence Analysis: A representative lot detected VGLUT1 in rat striatum (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Immunoautoradiography Analysis: A representative lot detected VGLUT1 in coronal sections of frontal rat brain (Herzog, E., et. al. (2004). Neuroscience. 123(4):983-1002) and rat brain sagittal sections (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Western Blotting Analysis: A representative lot detected VGLUT1 in BON cells stably expressing VGLUT1 (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
Research Category
Neuroscience
Neuroscience
Minőség
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected VGLUT1 in 20 µg of mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected VGLUT1 in 20 µg of mouse brain tissue lysate.
Cél megnevezése
~55 kDa observed; 61.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Fizikai forma
Format: Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide and 50% glycerol.
Unpurified
Tárolás és stabilitás
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Egyéb megjegyzések
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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