ABE1348
Anti-Histone H2A.Z Antibody (C-term)
from rabbit, purified by affinity chromatography
Szinonimák:
Histone H2A.Z, H2A/z
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(5)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
human
technika/technikák
ChIP: suitable (ChIP-seq)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... H2AFZ(3015)
Általános leírás
H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
Egyediség
Recognizes histone H2A.Z
wide range expected
Immunogén
KLH-conjugated linear peptide corresponding to region the near C-terminus of Human Histone H2A.Z.
Alkalmazás
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody (1μg per reaction) was used for ChIP using chromatin from HeLa and K562 cells.
ChIP-seq: A representative lot of this antibody (0.5 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.
Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.
Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.
ChIP-seq: A representative lot of this antibody (0.5 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.
Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.
Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.
This Anti-Histone H2A.Z Antibody (C-term) is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Immunocytochemistry and ELISA for the detection of Histone H2A.Z (C-term).
Minőség
Evaluated by Western Blotting using recombinant histones
Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.
Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.
Cél megnevezése
~16 kDa observed
Fizikai forma
Affinity purified rabbit polyclonal in PBS containing 0.05% azide and 0.05% ProClin 300.
Egyéb megjegyzések
Concentration: Please refer to lot specific datasheet.
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Skin Sens. 1
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Carlotta Zampieri et al.
Molecular oncology, 16(6), 1259-1271 (2021-12-18)
Somatic inactivation of p53 (TP53) mainly occurs as missense mutations that lead to the acquisition of neomorphic mutant protein forms. p53 mutants have been postulated to exert gain-of-function (GOF) effects, including promotion of metastasis and drug tolerance, which generally contribute
Gilda Stefanelli et al.
Cell reports, 36(7), 109551-109551 (2021-08-19)
Rapid removal of histone H2A.Z from neuronal chromatin is a key step in learning-induced gene expression and memory formation, but mechanisms underlying learning-induced H2A.Z removal are unclear. Anp32e was recently identified as an H2A.Z-specific histone chaperone that removes H2A.Z from
Anas Reda et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 49(8), 1285-1295 (2024-02-17)
Creating long-lasting memories requires learning-induced changes in gene expression, which are impacted by epigenetic modifications of DNA and associated histone proteins. Post-translational modifications (PTMs) of histones are key regulators of transcription, with different PTMs producing unique effects on gene activity
Firyal Ramzan et al.
Scientific reports, 10(1), 14331-14331 (2020-09-02)
Emerging evidence suggests that histone variants are novel epigenetic regulators of memory, whereby histone H2A.Z suppresses fear memory. However, it is not clear if altered fear memory can also modify risk for PTSD, and whether these effects differ in males
Klotilda Narkaj et al.
eNeuro, 5(5) (2018-11-13)
Memory formation is a protracted process that initially involves the hippocampus and becomes increasingly dependent on the cortex over time, but the mechanisms of this transfer are unclear. We recently showed that hippocampal depletion of the histone variant H2A.Z enhances
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással