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Merck
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Key Documents

12-371

Sigma-Aldrich

Normal Mouse IgG

Normal Mouse IgG Polyclonal Antibody control validated for use in Immunoprecipitation & Western Blotting.

Szinonimák:

Mouse IgG Antibody

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biológiai forrás

mouse

Minőségi szint

konjugátum

unconjugated

antitest forma

purified immunoglobulin

klón

polyclonal

gyártó/kereskedő neve

Upstate®

koncentráció

1 mg/mL

technika/technikák

immunoprecipitation (IP): suitable
western blot: suitable

izotípus

IgG

kiszállítva

wet ice

célzott transzláció utáni módosítás

unmodified

Általános leírás

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Normal mouse IgG was isolated from pooled normal mouse serum by fractionation and ion-exchange chromatography.

Alkalmazás

Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Minőség

Routinely evaluated by IP/WB as a negative non-specific IgG control.

Immunoprecipitation/Western Blot Assay:
4 µg of this lot was used as a negative non-specific IgG control in an immunoprecipitation/western blot assay.

Fizikai forma

Purified mouse IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide containing 30% glycerol. Liquid at -20°C.

Tárolás és stabilitás

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product centrifuge the original vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Jogi információk

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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