AB6016

Anti-F-actin-capping protein subunit alpha-1 Antibody

from rabbit, purified by affinity chromatography

• Szinonimák: capping protein (actin filament) muscle Z-line, alpha 1, CapZ alpha-1, F-actin-capping protein subunit alpha-1
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: polyclonal
• application: ICC; WB
• faj reaktivitás: pig, mouse, human, rat
• technika/technikák: immunocytochemistry: suitable; western blot: suitable
• citations: 6

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• tisztítva: affinity chromatography
• faj reaktivitás: pig, mouse, human, rat
• faj reaktivitás (homológia által előrejelzett): rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology), primate (based on 100% sequence homology), canine (based on 100% sequence homology), porcine (based on 100% sequence homology)
• technika/technikák: immunocytochemistry: suitable; western blot: suitable
• UniProt elérési szám: P52907
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: bovine ... Capza1(534656) ; dog ... Capza1(475857) ; human ... CAPZA1(829) ; mouse ... Capza1(12340) ; pig ... Capza1(100037957) ; rat ... Capza1(691149) ; rhesus monkey ... Capza1(705653)

Leírás

Általános leírás

F-actin capping protein subunit alpha (CapZ alpha) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. CapZ is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of CapZ’s subunits. All three alpha subunit isoforms are components of the WASH complex. Little is known about isoforms 1 and 2; however, isoform 3 is thought to be involved in spermatid morphogenesis and is exclusively observed within sperm and testis, with highest expression within the neck region of sperm.

Immunogén

GST-tagged recombinant protein corres-ponding to human F-actin-capping protein subunit alpha-1.

Alkalmazás

Detect F-actin-capping protein subunit alpha-1 using this Anti-F-actin-capping protein subunit alpha-1 Antibody validated for use in WB & IC.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western Blot (Snap i.d.) Analysis: 1 µg/mL from a previous lot detected F-actin-capping protein subunit alpha-1 on 10 µg of HeLa cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected F-actin-capping protein subunit alpha-1 in NIH/3T3, HeLa, and A431 cells.

Minőség

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit alpha-1 on 10 µg of HeLa cell lysate.

Cél megnevezése

~35 kDa

Fizikai forma

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Analízis megjegyzés

Control
HeLa cell lysate

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable