AB5032
Anti-Neural Cell Adhesion Molecule Antibody
Chemicon®, from rabbit
Szinonimák:
CD56 antigen, antigen recognized by monoclonal antibody 5.1H11
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
ELISA
IF
IHC
WB
IF
IHC
WB
faj reaktivitás:
mouse, rat, chicken, human
technika/technikák:
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
101
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity purified immunoglobulin
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
mouse, rat, chicken, human
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... NCAM1(4684)
Általános leírás
The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.
The previously assigned protein identifier P13592 has been merged into P13591. Full details can be found on the UniProt database.
Egyediség
Expected to cross-react with Chicken, Human, and rat.
Neural Cell Adhesion Molecule (NCAM). MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.
Immunogén
Highly purified chicken NCAM
Alkalmazás
Detect Neural Cell Adhesion Molecule using this Anti-Neural Cell Adhesion Molecule Antibody validated for use in ELISA, IF, IH, FUNC & WB.
Immunohistochemistry:
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Function blocking:
A previous lot of this antibody was used in Function blocking.
ELISA:
0.3 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Function blocking:
A previous lot of this antibody was used in Function blocking.
ELISA:
0.3 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Growth Cones & Axon Guidance
Minőség
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.
Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.
Cél megnevezése
MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa A breakdown product of 160 kDa may also be visible.
Fizikai forma
ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS containing no preservatives.
Tárolás és stabilitás
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analízis megjegyzés
Control
Neuraminidase-treated mouse brain membrane.
Neuraminidase-treated mouse brain membrane.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Taste bud cells of adult mice are responsive to Wnt/?-catenin signaling: implications for the renewal of mature taste cells.
Gaillard, D; Barlow, LA
Genesis (2000)
Maria S Skog et al.
Stem cell research & therapy, 7(1), 113-113 (2016-08-17)
In order to develop novel clinical applications and to gain insights into possible therapeutic mechanisms, detailed molecular characterization of human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) is needed. Neural cell adhesion molecule (NCAM, CD56) is a transmembrane glycoprotein modulating cell-cell
Protein kinase A activation promotes plasma membrane insertion of DCC from an intracellular pool: A novel mechanism regulating commissural axon extension.
Bouchard, JF; Moore, SW; Tritsch, NX; Roux, PP; Shekarabi, M; Barker, PA; Kennedy, TE
The Journal of Neuroscience null
Francisco Barrionuevo et al.
Developmental biology, 327(2), 301-312 (2009-01-07)
Sox9 and Sox8 are transcription factors expressed in embryonic and postnatal Sertoli cells of the mouse testis. Sox9 inactivation prior to the sex determination stage leads to complete XY sex reversal. In contrast, there is normal embryonic testis development in
Ladan Amin et al.
Journal of cell science, 129(20), 3878-3891 (2016-09-04)
The cellular prion protein (PrPC), encoded by the PRNP gene, is a ubiquitous glycoprotein, which is highly expressed in the brain. This protein, mainly known for its role in neurodegenerative diseases, is involved in several physiological processes including neurite outgrowth.
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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