AB5220
Anti-Growth Associated Protein-43 (GAP-43) Antibody
Chemicon®, from rabbit
Szinonimák:
Neuromodulin
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
mouse (high degree), rat, bovine, chicken, human
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... GAP43(2596)
Általános leírás
GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
Egyediség
Reacts to a lesser degree with rat, human, bovine and chicken. Other species have not been tested.
Recognizes Growth Associated Protein-43 (GAP-43).
Immunogen
Recombinant rat GAP-43 (complete sequence).
Alkalmazás
Anti-Growth Associated Protein-43 (GAP-43) Antibody is an antibody against Growth Associated Protein-43 (GAP-43) for use in ELISA, IC, IH, IP & WB.
Immunohistochemistry:
1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.
Immunocytochemistry:
1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF.
Immunoprecipitation:
1:100 dilution of a previous lot was used.
ELISA:
1:2,000 dilution of a previous lot was used.
1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.
Immunocytochemistry:
1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF.
Immunoprecipitation:
1:100 dilution of a previous lot was used.
ELISA:
1:2,000 dilution of a previous lot was used.
Minőség
Routinely evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.
Cél megnevezése
23.6 kDa
Fizikai forma
Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.01% sodium azide as a preservative.
Tárolás és stabilitás
Stable for up to 6 months at -20ºC in undiluted aliquots from date of receipt.
Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Analízis megjegyzés
Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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javasolt
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Analitikai tanúsítványok (COA)
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