71842
KOD Hot Start Master Mix
ready-to-use solution, Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., suitable for PCR
Szinonimák:
High fidelity master mix, Hot start PCR, Hot start master mix
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41105500
NACRES:
NA.55
Javasolt termékek
Minőségi szint
Forma
ready-to-use solution
használat
sufficient for 100 reactions
sufficient for 500 reactions
Jellemzők
Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart
gyártó/kereskedő neve
Novagen®
tárolási körülmény
OK to freeze
technika/technikák
PCR: suitable (2X PCR Master Mix for high fidelity)
PCR: suitable
bemenet
purified DNA
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
Általános leírás
KOD Hot Start Master Mix is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR, which utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. It contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure dNTPs, and reaction buffer with MgSO4. The mix is ideal for use in high-throughput PCR applications.
KOD Hot Start Master Mix is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR, which utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultra-pure deoxynucleotides, and reaction buffer with MgSO4. The monoclonal antibodies inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures.
Alkalmazás
KOD Hot Start Master Mix has been used:
- To yield PCR product for T7 endonuclease I (T7EI) assay.
- For PCR amplification of the DNA isolated from Chrysosporium multifidum, a fungus with antimicrobial activity.
- In the PCR amplification of cDNA.
- In two-step PCR for the amplification of the target sequence followed by its insertion into a vector.
Tulajdonságok és előnyök
- Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
- Amplifies genomic DNA templates up to 12 kbp
- Amplifies plasmid and lambda DNA template up to 21 kbp
- Eliminates mispriming and primer-dimer formation
- Convenient ambient-temperature setup compatible with automation
- KOD HotStart® Buffer ensures optimal PCR performance over a wide range of targets.
- Offers high specificity, fidelity and yield during PCR amplification
- Optimized for ease of use and data reproducibility
- Simplifies PCR set-up and saves time
- High consistency with minimal risk of contamination
*Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).
Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Komponensek
- 2 × 1.25 ml or 10 × 1.25 mL KOD Hot Start Master Mix
References:
Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem.66 (10), 2194. Fujii, S., et al. 1999. J. Mol.
Figyelmeztetés
Toxicity: Standard Handling (A)
Egység definíció
One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.
Egyéb megjegyzések
Simply add KOD HotStart® Master Mix to an equal volume of sample containing DNA template and primers. The final diluted reaction contains 1U KOD HotStart® DNA Polymerase per 50 μl reaction. The smaller available size provides sufficient master mix for 100 (50 μl scale), or 250 (20 μl scale) reactions, while the larger size is adequate for 500 (50 μl scale) or 1250 (20 μl scale) reactions.
Jogi információk
* Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
kapcsolódó termék
Product No.
Leírás
Árazás
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Yesenia Correa et al.
PloS one, 14(12), e0218837-e0218837 (2019-12-21)
The gut microbiota of insects is composed of a wide range of microorganisms which produce bioactive compounds that protect their host from pathogenic attack. In the present study, we isolate and identify the fungus Chrysosporium multifidum from the gut of
Adam S Dingens et al.
Cell host & microbe, 21(6), 777-787 (2017-06-06)
Precisely defining how viral mutations affect HIV's sensitivity to antibodies is vital to develop and evaluate vaccines and antibody immunotherapeutics. Despite great effort, a full map of escape mutants has not been delineated for an anti-HIV antibody. We describe a
Jesse D Bloom
Molecular biology and evolution, 31(8), 1956-1978 (2014-05-27)
All modern approaches to molecular phylogenetics require a quantitative model for how genes evolve. Unfortunately, existing evolutionary models do not realistically represent the site-heterogeneous selection that governs actual sequence change. Attempts to remedy this problem have involved augmenting these models
Michael B Doud et al.
Viruses, 8(6) (2016-06-09)
Influenza genes evolve mostly via point mutations, and so knowing the effect of every amino-acid mutation provides information about evolutionary paths available to the virus. We and others have combined high-throughput mutagenesis with deep sequencing to estimate the effects of
John A Burns et al.
Nucleic acids research, 38(22), 8178-8187 (2010-08-13)
O(6)-Methylguanine (O(6)-meG), which is produced in DNA following exposure to methylating agents, instructs human RNA polymerase II to mis-insert bases opposite the lesion during transcription. In this study, we examined the effect of O(6)-meG on transcription in human cells and
Cikkek
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
A PCR master mix leegyszerűsíti a PCR/RT-PCR-t olyan összetevőkkel, mint a DNS-polimeráz, dNTP-k, MgCl2 és puffer, amelyek kereskedelmi forgalomban vagy barkácsárukban kaphatók.
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
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