Összes fotó(1)
Fontos dokumentumok
23479
Nitrite/Nitrate Assay Kit, colorimetric
used for detection of nitric oxide metabolites
Szinonimák:
Nitrate/Nitrite Assay Kit, colorimetric
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12161503
NACRES:
NA.84
Javasolt termékek
![Nitrate Reductase (NAD[P]H) from Aspergillus niger lyophilized powder, ≥300 units/g solid](/deepweb/assets/sigmaaldrich/product/images/309/282/2a67ae4d-ca55-4f0b-96ec-34748ff8a21e/640/2a67ae4d-ca55-4f0b-96ec-34748ff8a21e.jpg)
![Nitrate Reductase (NAD[P]H) From Pichia Pastoris, recombinant freeze-dried protein glass](/deepweb/assets/sigmaaldrich/product/images/859/572/ae8d80df-c6d0-4cce-a526-35f8e21ca729/640/ae8d80df-c6d0-4cce-a526-35f8e21ca729.jpg)
Minőségi szint
használat
sufficient for 100 assays
alkalmasság
passes test for application
tárolási hőmérséklet
2-8°C
Related Categories
Alkalmazás
Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO′s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase.
The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM.
The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM.
Kit Components Only
Product No.
Leírás
- NaNO2 Standard Solution (100 mM) 1 bottle
- NaNO3 Standard Solution (100 mM) 1 bottle
- Buffer solution (20 mM, pH 7.6) 1 bottle
- Nitrate reductase (lyophilized) 1 bottle
- Enzyme Co-factor (lyophilized) 1 bottle
- Griess Reagent A 1 bottle
- Griess Reagent B 1 bottle
kapcsolódó termék
Product No.
Leírás
Árazás
Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Célzott szervek
Respiratory system
Tárolási osztály kódja
8A - Combustible corrosive hazardous materials
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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