233155

Cleland′s Reagent

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.

• Szinonimák: Cleland’s Reagent, DTT, Dithiothreitol, Dithiothreitol, DTT
• Tapasztalati képlet (Hill-képlet): C₄H₁₀O₂S₂
• CAS-szám: 3483-12-3
• Molekulatömeg: 154.25
• MDL-szám: MFCD00004877
• UNSPSC kód: 12161504
• NACRES: NA.25

Tulajdonságok

• Minőségi szint: 100
• leírás: Merck USA index - 14, 3376
• Teszt: ≥97% (titration)
• Forma: powder
• reakcióalkalmasság: reagent type: reductant
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze
• szín: white to off-white
• oldhatóság: water: 30 mg/mL; ethanol: soluble
• kation nyomok: heavy metals: ≤1 ppm; heavy metals: <1 ppm
• kiszállítva: ambient
• tárolási hőmérséklet: 2-8°C
• SMILES string: SC[C@H](O)[C@@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• Nemzetközi kémiai azonosító kulcs: VHJLVAABSRFDPM-IMJSIDKUSA-N

Leírás

Általános leírás

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.

DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.

Oxidized dithiothreitol: ≤0.5%.

Alkalmazás

• Cyclic nucleotide phosphodiesterase in Dictyostelium discoideum: interconversion of two enzyme forms.: Discusses the role of Cleland′s Reagent in differentiating forms of cyclic nucleotide phosphodiesterase, showcasing its utility in enzymatic studies and its broader implications in biochemistry research (Chassy BM, 1972).

Kiszerelés

Packaged under inert gas

Figyelmeztetés

Toxicity: Harmful (C)

Feloldás

Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Egyéb megjegyzések

Gailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Piktogramok: GHS05,GHS07
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H302,H315,H318
• Óvintézkedésre vonatkozó mondatok: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Veszélyességi osztályok: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable