05-908
Anti-RAD21 Antibody
Upstate®, from mouse
Szinonimák:
Anti-CDLS4, Anti-HR21, Anti-HRAD21, Anti-MCD1, Anti-MGS, Anti-NXP1, Anti-SCC1, Anti-hHR21
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
monoclonal
faj reaktivitás
human, mouse
gyártó/kereskedő neve
Upstate®
technika/technikák
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... RAD21(5885)
Általános leírás
Aka Scc1, part of the cohesin complex, Rad21 is a nuclear protein that associates with mitotic chromatin and is essential for the association of kinetochores with microtubules. Rad21/Scc1 s is cleaved by caspases, promoting apoptosis.
The protein encoded by the Rad21 gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase.
RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells
RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells
Egyediség
Rad21
Immunogen
GST fusion protein corresponding to residues 240-631 of human Rad21
Alkalmazás
Detect RAD21 with Anti-RAD21 Antibody (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Minőség
Routinely evaluated by immunoblot with HeLa nuclear extracts
Cél megnevezése
100-120kDa
Fizikai forma
Format: Purified
Protein G Purified
Prtotein G purified antibody in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4
Tárolás és stabilitás
Stable for 1 year at 2-8°C from date of receipt.
Analízis megjegyzés
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Próbálja ki a Termékválasztó eszköz. eszközt
javasolt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
CTCF controls expression and chromatin architecture of the human major histocompatibility complex class II locus.
Majumder, P; Boss, JM
Molecular and cellular biology null
Cohesin regulates MHC class II genes through interactions with MHC class II insulators.
Majumder, P; Boss, JM
Journal of immunology (Baltimore, Md. : 1950) (1950)
Scc1 sumoylation by Mms21 promotes sister chromatid recombination through counteracting Wapl.
Wu, N; Kong, X; Ji, Z; Zeng, W; Potts, PR; Yokomori, K; Yu, H
Genes & Development null
Johannes Buheitel et al.
The EMBO journal, 32(5), 666-676 (2013-01-31)
Faithful transmission of chromosomes during eukaryotic cell division requires sister chromatids to be paired from their generation in S phase until their separation in M phase. Cohesion is mediated by the cohesin complex, whose Smc1, Smc3 and Scc1 subunits form
Anders S Hansen et al.
eLife, 6 (2017-05-04)
Folding of mammalian genomes into spatial domains is critical for gene regulation. The insulator protein CTCF and cohesin control domain location by folding domains into loop structures, which are widely thought to be stable. Combining genomic and biochemical approaches we
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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