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Merck
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Fontos dokumentumok

05-829-AF647

Sigma-Aldrich

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 647 conjugate

clone DM1A, from mouse, ALEXA FLUOR 647

Szinonimák:

Tubulin alpha-1 chain, α-Tubulin

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
konjugátum:
ALEXA FLUOR 647
application:
ICC
klón:
DM1A, monoclonal
faj reaktivitás:
guinea pig, rat, porcine, human, gerbil, mouse, bovine, avian
citations:
4
technika/technikák:
immunocytochemistry: suitable

biológiai forrás

mouse

Minőségi szint

konjugátum

ALEXA FLUOR 647

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

DM1A, monoclonal

faj reaktivitás

guinea pig, rat, porcine, human, gerbil, mouse, bovine, avian

technika/technikák

immunocytochemistry: suitable

izotípus

IgG1

NCBI elérési szám

UniProt elérési szám

kiszállítva

wet ice

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... TUBA1A(7846)

Általános leírás

Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.

Egyediség

Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by clone DM1A has been mapped to the C-terminal 25 amino acids.

Immunogén

Epitope: C-terminal 25 amino acids.
Purified chicken brain α-Tubulin. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).

Alkalmazás

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 647 conjugate is an antibody against α-Tubulin for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected α-Tubulin in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Minőség

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-Tubulin in A431 cells.

Cél megnevezése

~50 kDa observed.

Fizikai forma

Protein G Purified
Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Egyéb megjegyzések

Concentration: Please refer to lot specific datasheet.

Jogi információk

ALEXA FLUOR is a trademark of Life Technologies

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Brandon Weissbourd et al.
Cell, 184(24), 5854-5868 (2021-11-26)
Jellyfish are radially symmetric organisms without a brain that arose more than 500 million years ago. They achieve organismal behaviors through coordinated interactions between autonomously functioning body parts. Jellyfish neurons have been studied electrophysiologically, but not at the systems level.
David J Schodt et al.
Biomedical optics express, 14(1), 429-440 (2023-01-27)
We describe a dedicated microscope for automated sequential localization microscopy which we term Sequential Super-resolution Microscope (SeqSRM). This microscope automates precise stage stabilization on the order of 5-10 nanometers and data acquisition of all user-selected cells on a coverslip, limiting
Farah Haque et al.
Nature cell biology, 24(7), 1088-1098 (2022-06-21)
A long-established strategy for transcription regulation is the tethering of transcription factors to cellular membranes. By contrast, the principal effectors of Hedgehog signalling, the GLI transcription factors, are regulated by microtubules in the primary cilium and the cytoplasm. How GLI
Luqman O Awoniyi et al.
Journal of cell science, 136(15) (2023-07-07)
Successful B cell activation, which is critical for high-affinity antibody production, is controlled by the B cell antigen receptor (BCR). However, we still lack a comprehensive protein-level view of the very dynamic multi-branched cellular events triggered by antigen binding. Here

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