05-690
Anti-HP1γ Antibody, clone 42s2
clone 42s2, Upstate®, from mouse
Szinonimák:
HP1 gamma, HP1 gamma homolog, Heterochromatin protein 1 homolog gamma, Modifier 2 protein, chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox homolog 3 (HP1 gamma homolog, Drosophila), heterochromatin protein HP1 gamma, heterochro
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
42s2, monoclonal
application:
ChIP
IHC
WB
IHC
WB
faj reaktivitás:
human, mouse
technika/technikák:
ChIP: suitable
immunohistochemistry: suitable
western blot: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
60
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
42s2, monoclonal
faj reaktivitás
human, mouse
gyártó/kereskedő neve
Upstate®
technika/technikák
ChIP: suitable
immunohistochemistry: suitable
western blot: suitable
izotípus
IgG1
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... CBX3(11335)
Általános leírás
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated
Egyediség
Reactivity with other species has not been determined.
This antibody recognizes HP1γ protein, Mr ~25 kDa.
Immunogén
GST fusion protein corresponding to residues 17-173 of human HP1γ protein. Clone 42s2.
Alkalmazás
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect HP1γ positive cells in breast tissue.
Immunoprecipitation/Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked chromatin.
This antibody has been reported by an independent laboratory to detect HP1γ positive cells in breast tissue.
Immunoprecipitation/Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked chromatin.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Use Anti-HP1γ Antibody, clone 42s2 (Mouse Monoclonal Antibody) validated in ChIP, IHC, WB to detect HP1γ also known as Heterochromatin protein 1 homolog gamma.
Minőség
Routinely evaluated by western blot in acid-extracted HeLa cell lysates (Catalog #13-112).
Western Blot Analysis:
0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell lysates (Catalog # 13-112).
Western Blot Analysis:
0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell lysates (Catalog # 13-112).
Cél megnevezése
25 kDa
Fizikai forma
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20ºC.
Tárolás és stabilitás
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analízis megjegyzés
Control
HeLa acid extracts, HEK 293 nuclear extracts.
HeLa acid extracts, HEK 293 nuclear extracts.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Due to license agreement restrictions, this product cannot be purchased for resale.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Thang Van Nguyen et al.
The Journal of experimental medicine, 204(6), 1453-1461 (2007-05-31)
Tumor suppressor p53-dependent apoptosis is critical in suppressing tumorigenesis. Previously, we reported that DNA double-strand breaks (DSBs) at the V(D)J recombination loci induced genomic instability in the developing lymphocytes of nonhomologous end-joining (NHEJ)-deficient, p53-deficient mice, which led to rapid lymphomagenesis.
A NF-?B-dependent dual promoter-enhancer initiates the lipopolysaccharide-mediated transcriptional activation of the chicken lysozyme in macrophages.
Witham, J; Ouboussad, L; Lefevre, PF
Testing null
Eva Bártová et al.
Differentiation; research in biological diversity, 76(1), 24-32 (2007-06-19)
Human embryonic stem cells (hES) are unique in their pluripotency and capacity for self-renewal. Therefore, we have studied the differences in the level of chromatin condensation in pluripotent and all-trans retinoic acid-differentiated hES cells. Nuclear patterns of the Oct4 (6p21.33)
Rosa M Marion et al.
Cell stem cell, 4(2), 141-154 (2009-02-10)
Telomere shortening is associated with organismal aging. iPS cells have been recently derived from old patients; however, it is not known whether telomere chromatin acquires the same characteristics as in ES cells. We show here that telomeres are elongated in
Meike Wiese et al.
Epigenetics & chromatin, 12(1), 21-21 (2019-04-04)
Stem cell differentiation involves major chromatin reorganisation, heterochromatin formation and genomic relocalisation of structural proteins, including heterochromatin protein 1 gamma (HP1γ). As the principal reader of the repressive histone marks H3K9me2/3, HP1 plays a key role in numerous processes including
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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