05-1530-M

Anti-Importin beta-1 Antibody, clone 3H14

clone 3H14, from mouse

• Szinonimák: Karyopherin subunit beta-1, Nuclear factor P97, importin 1, importin 90, importin beta-1 subunit, karyopherin (importin) beta 1, karyopherin beta 1
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 100
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: 3H14, monoclonal
• faj reaktivitás: human
• technika/technikák: immunocytochemistry: suitable; western blot: suitable
• izotípus: IgG1κ
• NCBI elérési szám: NP_002256.2
• UniProt elérési szám: Q14974
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... KPNB1(3837)

Leírás

Általános leírás

Importin beta-1 (also known as Importin subunit beta-1) facilitates nuclear protein import. This occurs either though association with a nuclear localization signal (NLS) and an adapter protein , such as an importin-alpha subunit, which which binds to nuclear, or directly where the protein acts as autonomous nuclear transport receptor.
Import of macromolecules containing a NLS requires a heterodimer of importin alpha and beta subunits also called as karyopherins. Importin alpha binds the cytoplasmic NLS-containing protein and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. Movement through the nuclear pore is mediated by the small GTP binding protein Ran via an energy requiring mechanism. Using nucleotide triphosphates and Ran, the nuclear transport complex moves into the nuclear pore and the importin subunits dissociate. Importin alpha and its passenger protein enter the nucleoplasm while importin beta remains at the pore.

Egyediség

This antibody recognizes Importin beta-1.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human Importin beta-1.

Alkalmazás

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-Importin beta-1 Antibody, clone 3H14 (Mouse Monoclonal Antibody) validated in WB, ICC to detect Importin beta-1 also known as Karyopherin subunit beta-1, Nuclear factor P97.

Minőség

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Importin beta-1 on 10 µg of HeLa cell lysate.

Cél megnevezése

~ 97 kDa

Fizikai forma

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Analízis megjegyzés

Control
HeLa cell lysate

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable