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Merck

03285

Sigma-Aldrich

Cell Counting Kit

sufficient for 500 tests

Sinónimos:

Fluorescent Cell Counter

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About This Item

UNSPSC Code:
12352116
eCl@ss:
32161000
NACRES:
NA.32

form

liquid

usage

sufficient for 500 tests

storage temp.

−20°C

General description

Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.

Application

Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.

Features and Benefits

Cell Counting Kit has the following advantages:

  • It doesn’t require any radioisotopes to function.
  • No solubilization procedure is needed.
  • Results are highly reproducible and accurate.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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MicroRNA (miR)-217 has been reported to participate in carcinogenesis and tumor progression in several cancers; however, its expression and biological functions in colorectal cancer (CRC) are still unclear. The present study demonstrated that miR-217 expression was significantly higher in matched

Artículos

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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