C1359

Calcein AM solution

≥90% purity (HPLC), solution

• Fórmula empírica (notación de Hill): C₄₆H₄₆N₂O₂₃
• Número de CAS: 148504-34-1
• Peso molecular: 994.86
• Número CE: 200-664-3
• Número MDL: MFCD05861516
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24892405
• NACRES: NA.47

Propiedades

• Nombre del producto: Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
• Nivel de calidad: 100
• Ensayo: ≥90% (HPLC)
• Formulario: solution
• concentración: 4 mM in DMSO
• técnicas: titration: suitable
• color: colorless; white to off-white
• λmáx.: 491-497 nm
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: −20°C
• cadena SMILES: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
• Clave InChI: XKFSBWQWNMZWFA-UHFFFAOYSA-N

Descripción

Aplicación

In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.

Acciones bioquímicas o fisiológicas

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.

Fluorescent cell permeable derivative of calcein.

Forma física

Solution in 0.4% Calcein AM and 99.6% DMSO.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): 185.0 °F - closed cup
• Punto de inflamabilidad (°C): 85 °C - closed cup
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)