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Merck

G5160

Sigma-Aldrich

β-Galactosidase from Aspergillus oryzae

≥8.0 units/mg solid

Sinónimos:

β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae

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About This Item

Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

solid

specific activity

≥8.0 units/mg solid

contains

dextrin as extender

storage temp.

−20°C

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General description

β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.

Application

β-Galactosidase from Aspergillus oryzae has been used:
  • in in vitro nuclear magnet resonance (NMR)
  • as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
  • to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
  • for enzymatic hydrolysis of lactose
  • for reversed-phase (RP) adsorption
  • in the hydrolysis of whey lactose

Biochem/physiol Actions

β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Unit Definition

1 U hydrolysiert 1.0 μmol o-Nitrophenyl β-D-Galactosid zu o-Nitrophenol und D-Galactose pro Minute beim angegebenen pH- und Temperatur-Wert, falls nicht anders erwähnt.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Martin Dragosits et al.
Applied microbiology and biotechnology, 98(8), 3553-3567 (2013-09-17)
Galactosidases are widespread enzymes that are used for manifold applications, including production of prebiotics, biosynthesis of different transgalactosylated products, improving lactose tolerance and in various analytical approaches. The nature of these applications often require galactosidases to be present in a
C M Seubert et al.
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Modular process for the flexible synthesis of magnetic beads-Process and product validation
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Journal of Applied Polymer Science, 112, 2366-2373 (2009)
Jian-Xin Yu et al.
NMR in biomedicine, 21(7), 704-712 (2008-02-22)
Gene therapy has emerged as a promising strategy for treatment of various diseases. However, widespread implementation is hampered by difficulties in assessing the success of transfection in the target tissue and the longevity of gene expression. Thus, there is increasing
Hydrolysis of whey lactose by immobilized β-Galactosidase
Marcela Panaro Mariotti; Hideko Yamanaka; Angela Regina Araujo; Henrique Celso Trevisan
Brazilian Archives of Biology and Technology, 51 (2008)

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