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B4252

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

chromogenic, ≥98%, powder

Sinónimos:

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Fórmula empírica (notación de Hill):
C14H15BrClNO6
Número de CAS:
7240-90-6
Peso molecular:
408.63
Beilstein/REAXYS Number:
1402009
EC Number:
230-640-8
MDL number:
MFCD00005666
UNSPSC Code:
12352204
PubChem Substance ID:
24891761
NACRES:
NA.83

product name

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%, powder

Quality Level

200

assay

≥98%

form

powder

solubility

DMF: methanol (1:1): 50 mg/mL

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

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Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

related product

Referencia del producto
Descripción
Precios

94433

β-Galactosidase stain

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000387179
0000386320
0000386322
0000386309
0000378919

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An acute increase in blood flow triggers flow-mediated dilation (FMD), which is mainly mediated by endothelial nitric oxide synthase (eNOS). A long-term increase in blood flow chronically enlarges the arterial lumen, a process called arteriogenesis. In several common human diseases
Yibing Wang et al.
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Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a
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