G4155
β-Galactosidasa from Escherichia coli
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
Sinónimos:
β-D-galactósido galactohidrolasa, Lactasa
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About This Item
Número de CAS:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Productos recomendados
form
aqueous glycerol suspension
Quality Level
specific activity
≥500 units/mg protein (biuret)
mol wt
465 kDa
storage temp.
−20°C
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Application
β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.
Biochem/physiol Actions
βLa β-galactosidasa hidroliza la lactosa en sus componentes monosacáridos, glucosa y galactosa. También cataliza la transglucosilación de la glucosa en alolactosa, el inductor de la β-galactosidasa, en un bucle de retroalimentación.
Physical properties
Peso molecular del tetrámero 465 kDa (subunidades de 116,3 kDa cada una)
Unit Definition
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Physical form
Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4
Other Notes
Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).
Inhibitor
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Substrate
Referencia del producto
Descripción
Precios
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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