G3153

β-Galactosidasa from Escherichia coli

lyophilized powder, ≥500 units/mg protein

• Sinónimos: β-D-galactósido galactohidrolasa, Lactasa
• Número CAS: 9031-11-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-864-1
• MDL number: MFCD00155434
• Número CE: 3.2.1.23 (BRENDA, IUBMB)
• Specific activity: ≥500 units/mg protein
• Recombinant: expressed in E. coli

Propiedades

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 465 kDa
• storage temp.: −20°C

Descripción

General description

Peso molecular del tetrámero 465 kDa (subunidades de 116,3 kDa cada una)

β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.

Application

β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.

β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).

Biochem/physiol Actions

β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.

βLa β-galactosidasa hidroliza la lactosa en sus componentes monosacáridos, glucosa y galactosa. También cataliza la transglucosilación de la glucosa en alolactosa, el inductor de la β-galactosidasa, en un bucle de retroalimentación.

Packaging

Package size based on protein content

Physical form

Stabilized with phosphate buffer and sucrose

Other Notes

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type N95 (US)
• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1