R6148
RNase A solution
Sinonimo/i:
GenElute™ RNAse A
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About This Item
Codice UNSPSC:
12352204
NACRES:
NA.52
Prodotti consigliati
Origine biologica
bovine pancreas
Forma fisica
liquid
Attività specifica
1500 units/mL
3000 units/mL
Concentrazione
20 mg/mL in Tris buffer
tecniche
DNA purification: suitable
Temperatura di conservazione
room temp
Informazioni sul gene
bovine ... RNASE1(282340) , RNASE1(280930)
Descrizione generale
Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute™ kits.
For sample preparation in GenElute™ kits.
Applicazioni
RNase A solution has been used:
- in apoptosis assay of Hep-2 cells
- for the colorimetric quantification of methylated DNA
- for DNA and RNA extraction
- in DNA laddering analysis
- in cell cycle analysis
- as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry
Azioni biochim/fisiol
Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.
Nota sulla preparazione
For usage requirements, please refer to the kit procedure.
Note legali
GenElute is a trademark of Sigma-Aldrich Co. LLC
Preparazione del campione
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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