NA0600

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit

1 kit sufficient for 5 preparations

• Sinonimo/i: Gen Elute, GenElute Endotoxin-Free Plasmid Kit
• Codice UNSPSC: 41105501
• NACRES: NA.52

Proprietà

• impiego: kit sufficient for 5 preparations
• Caratteristiche più verdi: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Categoria alternativa più verde: , Aligned
• Temperatura di conservazione: 15-25°C

Descrizione

Descrizione generale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

Applicazioni

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

Caratteristiche e vantaggi

• Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
• Only 90 minutes from pelleted cells to purified plasmid
• Vacuum format with no ethanol precipitation required

Altre note

For additional information, please see www.sigma-aldrich.com/genelutehp.

Note legali

GenElute is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS08,GHS05,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H226,H290,H302 + H332,H315,H319,H334,H336,H372
• Consigli di prudenza: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
• Organi bersaglio: Central nervous system, Lungs
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Punto d’infiammabilità (°F): 77.0 °F - closed cup
• Punto d’infiammabilità (°C): 25 °C - closed cup