Passa al contenuto
Merck
Tutte le immagini(4)

Documenti

NA2020

Sigma-Aldrich

GenElute™ Blood Genomic DNA Kit

sufficient for 350 purifications

Sinonimo/i:

Blood Genomic DNA, Gen Elute

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(4)

About This Item

Codice UNSPSC:
41105501
NACRES:
NA.52

impiego

sufficient for 350 purifications

Livello qualitativo

200

tecniche

DNA purification: suitable

Temperatura di conservazione

15-25°C
room temp

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Applicazioni

The purified genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning

Caratteristiche e vantaggi

  • Starting material: Up to 200 μl of fresh or aged blood
  • Expected yield: Up to 10 μg
  • Elution volume: 400 μl
  • Time required: <40 min
  • A260/A280 ratio: 1.6 - 1.9
  • Compatible with many anticoagulants, including EDTA, Heparin, and Sodium Citrate

Principio

The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.

The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Altre note

For additional information, please see www.sigma-aldrich.com/genomicdna.

Note legali

GenElute is a trademark of Sigma-Aldrich Co. LLC

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

P2308

Proteinasi K, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Bromuro di etidio, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarosio, BioReagent, for molecular biology, low EEO

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tampone Tris-borato-EDTA, 5× Concentrate

D9780

Lambda DNA Hind III Digest, for DNA electrophoresis

R6148

RNase A solution

NA2010

GenElute™ Blood Genomic DNA Kit, sufficient for 70 purifications

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

SRE0005

Proteinasi K, Reagents designed and manufactured under current ISO 13485 certification.

Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Avvertenze

Danger

Indicazioni di pericolo

H290,H302,H315,H318,H334,H335,H410

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 2

1 of 2

Nalgene&#174; bottles, style 2126 capacity 4000&#160;mL

B7910

Nalgene® bottles, style 2126

GenElute&#8482; Plant Genomic DNA Miniprep Kit sufficient for 350&#160;purifications

Sigma-Aldrich

G2N350

GenElute™ Plant Genomic DNA Miniprep Kit

Miri Cohen-Zinder et al.
Physiological genomics, 48(5), 367-376 (2016-03-20)
Ecological and economic concerns drive the need to improve feed utilization by domestic animals. Residual feed intake (RFI) is one of the most acceptable measures for feed efficiency (FE). However, phenotyping RFI-related traits is complex and expensive and requires special
S K Sahu et al.
Oncogenesis, 5, e225-e225 (2016-05-10)
Tumor suppressor p53 is a critical player in the fight against cancer as it controls the cell cycle check point, apoptotic pathways and genomic stability. It is known to be the most frequently mutated gene in a wide variety of
Disha Bhange et al.
Frontiers in microbiology, 12, 779472-779472 (2021-12-14)
In a multicentric, observational, investigator-blinded, and longitudinal clinical study of 764 ART-naïve subjects, we identified nine different promoter variant strains of HIV-1 subtype C (HIV-1C) emerging in the Indian population, with some of these variants being reported for the first
Anika Daing et al.
Journal of periodontal & implant science, 42(5), 151-157 (2012-11-28)
Cyclooxygenase (COX) enzyme catalyzes the production of prostaglandins, which are important mediators of tissue destruction in periodontitis. Single nucleotide polymorphisms of COX2 enzyme have been associated with increasing susceptibility to inflammatory diseases. The present study evaluates the association of two
C Castellini et al.
Theriogenology, 78(4), 715-723 (2012-06-19)
There is increasing evidence that the particulate fraction of seminal plasma plays an important role in reproduction of several mammalian species. However, the origin and role of these granules in the physiology of rabbit spermatozoa is partially unknown. The aim
Visualizza tutti i documenti correlati

Protocolli

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.