70856
RNase A Solution
Convenient solution for selective degradation of RNA
Sinonimo/i:
RNase A Enzyme
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 91.30 |
Informazioni su questo articolo
NACRES:
NA.54
UNSPSC Code:
12352202
Concentration:
10 mg/mL
CHF 91.30
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartiform
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
10 mg/mL
technique(s)
DNA purification: suitable
shipped in
ambient
storage temp.
10-30°C
General description
RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA[1] purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.
Application
RNase A Solution has been used:
- in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
- in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays[2]
- to treat control adult and embryonic thymi cells during staining
- to remove RNA from affibody–mRNA fusion clones before clone screening
Biochem/physiol Actions
RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Questo articolo | |||
|---|---|---|---|
| technique(s) DNA purification: suitable | technique(s) - | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable |
| concentration 10 mg/mL | concentration - | concentration - | concentration 20 mg/mL in Tris buffer |
| form solution | form lyophilized solid | form solution | form liquid |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
| storage temp. 10-30°C | storage temp. −20°C | storage temp. −20°C | storage temp. room temp |
| storage condition OK to freeze | storage condition OK to freeze, desiccated | storage condition - | storage condition - |
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Danger
hcodes
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Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 2
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